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Alzheimer's amyloid-β and tau protein accumulation is associated with decreased expression of the LDL receptor-associated protein in human brain tissue

Citation

Shepherd, CE and Affleck, AJ and Bahar, AY and Carew-Jones, F and Gregory, G and Small, DH and Halliday, GM, Alzheimer's amyloid-β and tau protein accumulation is associated with decreased expression of the LDL receptor-associated protein in human brain tissue, Brain and Behavior Article e01672. ISSN 2162-3279 (2020) [Refereed Article]


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Copyright 2020 2020 The Authors. Brain and Behavior published by Wiley Periodicals LLC Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1002/brb3.1672

Abstract

Introduction: One of the major neuropathological features of Alzheimer's disease (AD) is the accumulation of amyloid-β (Aβ) protein in the brain. Evidence suggests that the low-density lipoprotein receptor-associated protein (RAP) binds strongly to Aβ and enhances its cellular uptake and that decreased RAP expression correlates with increased Aβ production in animal models of AD.

Methods: The current study examined whether RAP levels change in AD human brain tissue and whether they are related to the amount of AD pathology. RAP and NeuN levels were determined by Western blot, while low-density lipoprotein receptor-related protein 1 (LRP1), tau and Aβ levels were determined by ELISA in the temporal cortex of 17 AD and 16 control cases.

Results: An increase in total Aβ and insoluble and soluble tau protein was observed in AD brain tissue. In contrast, RAP levels were significantly decreased in AD brain tissue compared to controls. Correlation analysis revealed that levels of RAP correlated with both total Aβ and soluble and insoluble tau levels. Neither LRP1 nor NeuN levels were significantly altered in AD brain tissue homogenates and did not correlate with Aβ or tau protein levels.

Conclusion: Reduction in RAP may contribute to the accumulation and aggregation of Aβ in the AD brain.

Item Details

Item Type:Refereed Article
Keywords:Alzheimer's disease, ELISA, amyloid-β, receptor-associated protein, tau
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Small, DH (Professor David Small)
ID Code:139817
Year Published:2020
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-07-07
Last Modified:2020-08-04
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