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Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation

journal contribution
posted on 2023-05-20, 14:05 authored by Dorantes-Aranda, JJ, Hayashi, A, Alison TurnbullAlison Turnbull, Jolley, JYC, Harwood, DT, Gustaaf HallegraeffGustaaf Hallegraeff
Background

Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money.

Objective

The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster.

Methods

The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4).

Results

The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences.

Conclusions

The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster.

Highlights

The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.

Funding

Fisheries Research & Development Corporation

History

Publication title

Journal of AOAC International

Volume

103

Pagination

784-791

ISSN

1060-3271

Department/School

Institute for Marine and Antarctic Studies

Publisher

Aoac International

Place of publication

481 North Fredrick Ave, Ste 500, Gaithersburg, USA, Md, 20877-2504

Rights statement

Copyright 2020 AOAC International

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture rock lobster; Wild caught rock lobster

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