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Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation

Citation

Dorantes-Aranda, JJ and Hayashi, A and Turnbull, AR and Jolley, JYC and Harwood, DT and Hallegraeff, GM, Detection of paralytic shellfish toxins in southern rock lobster Jasus edwardsii using the qualitative NeogenTM lateral flow immunoassay: single-laboratory validation, Journal of AOAC International, 103, (3) pp. 784-791. ISSN 1060-3271 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 AOAC International

DOI: doi:10.1093/jaocint/qsz029

Abstract

Background

Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money.

Objective

The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster.

Methods

The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4).

Results

The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences.

Conclusions

The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster.

Highlights

The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.

Item Details

Item Type:Refereed Article
Keywords:PST test kit validation, rock lobster
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fisheries management
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - wild caught
Objective Field:Wild caught rock lobster
UTAS Author:Dorantes-Aranda, JJ (Dr Juan Dorantes Aranda)
UTAS Author:Hayashi, A (Ms Aiko Hayashi)
UTAS Author:Turnbull, AR (Ms Alison Turnbull)
UTAS Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
ID Code:138851
Year Published:2020
Deposited By:Ecology and Biodiversity
Deposited On:2020-05-04
Last Modified:2020-12-08
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