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Sugar beet (Beta vulgaris) guard cells responses to salinity stress: a proteomic analysis

Citation

Rasouli, F and Kiani-Pouya, A and Li, L and Zhang, H and Chen, Z and Hedrich, R and Wilson, R and Shabala, S, Sugar beet (Beta vulgaris) guard cells responses to salinity stress: a proteomic analysis, International Journal of Molecular Sciences, 21, (7) Article 2331. ISSN 1422-0067 (2020) [Refereed Article]


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Copyright 2020 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms21072331

Abstract

Soil salinity is a major environmental constraint affecting crop growth and threatening global food security. Plants adapt to salinity by optimizing the performance of stomata. Stomata are formed by two guard cells (GCs) that are morphologically and functionally distinct from the other leaf cells. These microscopic sphincters inserted into the wax-covered epidermis of the shoot balance CO2 intake for photosynthetic carbon gain and concomitant water loss. In order to better understand the molecular mechanisms underlying stomatal function under saline conditions, we used proteomics approach to study isolated GCs from the salt-tolerant sugar beet species. Of the 2088 proteins identified in sugar beet GCs, 82 were differentially regulated by salt treatment. According to bioinformatics analysis (GO enrichment analysis and protein classification), these proteins were involved in lipid metabolism, cell wall modification, ATP biosynthesis, and signaling. Among the significant differentially abundant proteins, several proteins classified as "stress proteins" were upregulated, including non-specific lipid transfer protein, chaperone proteins, heat shock proteins, inorganic pyrophosphatase 2, responsible for energized vacuole membrane for ion transportation. Moreover, several antioxidant enzymes (peroxide, superoxidase dismutase) were highly upregulated. Furthermore, cell wall proteins detected in GCs provided some evidence that GC walls were more flexible in response to salt stress. Proteins such as L-ascorbate oxidase that were constitutively high under both control and high salinity conditions may contribute to the ability of sugar beet GCs to adapt to salinity by mitigating salinity-induced oxidative stress.

Item Details

Item Type:Refereed Article
Keywords:guard cells, stomata, sugar beet, salt stress, proteomic
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Proteomics and intermolecular interactions (excl. medical proteomics)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Rasouli, F (Ms Fatemeh Rasouli)
UTAS Author:Kiani-Pouya, A (Dr Ali Kiani-Pouya)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:138705
Year Published:2020
Web of Science® Times Cited:1
Deposited By:Central Science Laboratory
Deposited On:2020-04-23
Last Modified:2020-12-08
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