eCite Digital Repository

In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS-NIR slow-induced conformational pigment bed changes

Citation

Van Wittenberghe, S and Alonso, L and Malenovsky, Z and Moreno, J, In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS-NIR slow-induced conformational pigment bed changes, Photosynthesis Research, 142, (3) pp. 283-305. ISSN 0166-8595 (2019) [Refereed Article]


Preview
PDF
27Mb
  

Copyright Statement

© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

DOI: doi:10.1007/s11120-019-00664-3

Abstract

Regulated heat dissipation under excessive light comprises a complexity of mechanisms, whereby the supramolecular light-harvesting pigment–protein complex (LHC) shifts state from light harvesting towards heat dissipation, quenching the excess of photo-induced excitation energy in a non-photochemical way. Based on whole-leaf spectroscopy measuring upward and downward spectral radiance fluxes, we studied spectrally contiguous (hyperspectral) transient time series of absorbance A(λ,t) and passively induced chlorophyll fluorescence F(λ,t) dynamics of intact leaves in the visible and near-infrared wavelengths (VIS–NIR, 400–800 nm) after sudden strong natural-like illumination exposure. Besides light avoidance mechanism, we observed on absorbance signatures, calculated from simultaneous reflectance R(λ,t) and transmittance T(λ,t) measurements as A(λ,t) = 1 - R(λ,t) - T(λ,t), major dynamic events with specific onsets and kinetical behaviour. A consistent well-known fast carotenoid absorbance feature (500-570 nm) appears within the first seconds to minutes, seen from both the reflected (backscattered) and transmitted (forward scattered) radiance differences. Simultaneous fast Chl features are observed, either as an increased or decreased scattering behaviour during quick light adjustment consistent with re-organizations of the membrane. The carotenoid absorbance feature shows up simultaneously with a major F decrease and corresponds to the xanthophyll conversion, as quick response to the proton gradient build-up. After xanthophyll conversion (t = 3 min), a kinetically slower but major and smooth absorbance increase was occasionally observed from the transmitted radiance measurements as wide peaks in the green (∼ 550 nm) and the near-infrared (∼ 750 nm) wavelengths, involving no further F quenching. Surprisingly, in relation to the response to high light, this broad and consistent VIS–NIR feature indicates a slowly induced absorbance increase with a sigmoid kinetical behaviour. In analogy to sub-leaf-level observations, we suggest that this mechanism can be explained by a structure-induced low-energy-shifted energy redistribution involving both Car and Chl. These findings might pave the way towards a further non-invasive spectral investigation of antenna conformations and their relations with energy quenching at the intact leaf level, which is, in combination with F measurements, of a high importance for assessing plant photosynthesis in vivo and in addition from remote observations.

Item Details

Item Type:Refereed Article
Keywords:absorbance shift, absorbed photosynthetic active radiation (APAR), controlled heat dissipation, hyperspectral remote sensing, non-photochemical quenching (NPQ), passive chlorophyll a fluorescence , pigment– protein dynamics
Research Division:Engineering
Research Group:Geomatic engineering
Research Field:Photogrammetry and remote sensing
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Malenovsky, Z (Dr Zbynek Malenovsky)
ID Code:136282
Year Published:2019
Web of Science® Times Cited:6
Deposited By:Geography and Spatial Science
Deposited On:2019-12-12
Last Modified:2020-05-19
Downloads:9 View Download Statistics

Repository Staff Only: item control page