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Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein

Citation

Ventura, T and Nguyen, C and Fitzgibbon, QP and Abramov, T and Smith, GG and Elizur, A, Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein, Scientific Reports, 9, (1) Article 2847. ISSN 2045-2322 (2019) [Refereed Article]


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Copyright 2019 the authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/s41598-019-39173-7

Abstract

Literature on the cuticle formation in larval stages of the diverse group of decapod crustaceans is lacking, as opposed to a wealth of knowledge in several insect groups. Here we provide the first glimpse of the cuticular organisation in larvae of the eastern spiny lobster Sagmariasus verreauxi. A bioinformatic approach applied to S. verreauxi transcriptome through metamorphosis identified for the first time a small secreted protein with multiple isoforms that is highly expressed in crustacean larvae. This protein, named crustacean larval factor (Clf) shares structural characteristics with insect follicle cell protein 3 (FCP3), an insect-specific, rapidly evolving protein, with spatial-temporal regulated expression that is restricted to follicular cells during the production of the vitellin coat. Furthermore, we identified the FCP3 domain in additional structural proteins in multiple arthropod groups. Recombinant Clf inhibited in vitro calcium carbonate crystalline precipitation, in keeping with the finding that the spiny lobster larval cuticle is mainly composed of amorphous calcium carbonate. In addition, the recombinant Clf was shown to bind chitosan. Taken together, this research identifies two novel structural domains with lineage-specific expansion across arthropods. In crustaceans, Clf is found predominantly in larvae and the spatial-temporal regulated FCP3 factor occurs as a domain identified in multiple structural proteins across arthropods. Given the shared ten cysteines backbone between the Clf and FCP domains, a shared evolution is suggested and should be further explored.

Item Details

Item Type:Refereed Article
Keywords:lobster, cuticle, bioinformatics
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish physiology and genetics
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture rock lobster
UTAS Author:Fitzgibbon, QP (Associate Professor Quinn Fitzgibbon)
UTAS Author:Smith, GG (Professor Gregory Smith)
ID Code:131749
Year Published:2019
Funding Support:Australian Research Council (DP160103320)
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2019-04-03
Last Modified:2020-08-06
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