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Propagation and Purification of Ectromelia Virus
journal contribution
posted on 2023-05-20, 02:20 authored by Chaudhri, G, Kaladimou, G, Pratikshya PandeyPratikshya Pandey, Gunasegaran KarupiahGunasegaran KarupiahEctromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Mousepox makes an excellent small animal model for smallpox to investigate pathogenesis, vaccine and antiviral agent testing, host-virus interactions, and immune and inflammatory responses. The availability of a wide variety of inbred, congenic, and gene-knockout mice allows detailed analyses of the host response. ECTV mutant viruses lacking one or more genes encoding immunomodulatory proteins are being used in numerous studies in conjunction with wild-type or gene-knockout mice to study the functions of these genes in host-virus interactions. The methods used for propagation of ECTV in cell culture, purification, and quantification of infectious particles through viral plaque assay are described.
History
Publication title
Current Protocols in MicrobiologyVolume
51ISSN
1934-8525Department/School
Tasmanian School of MedicinePublisher
John WileyPlace of publication
United StatesRights statement
Copyright 2018 John Wiley & Sons, Inc.Repository Status
- Restricted