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Propagation and Purification of Ectromelia Virus

Citation

Chaudhri, G and Kaladimou, G and Pandey, P and Karupiah, G, Propagation and Purification of Ectromelia Virus, Current Protocols in Microbiology, 51, (1) ISSN 1934-8525 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 John Wiley & Sons, Inc.

DOI: doi:10.1002/cpmc.65

Abstract

Ectromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Mousepox makes an excellent small animal model for smallpox to investigate pathogenesis, vaccine and antiviral agent testing, host-virus interactions, and immune and inflammatory responses. The availability of a wide variety of inbred, congenic, and gene-knockout mice allows detailed analyses of the host response. ECTV mutant viruses lacking one or more genes encoding immunomodulatory proteins are being used in numerous studies in conjunction with wild-type or gene-knockout mice to study the functions of these genes in host-virus interactions. The methods used for propagation of ECTV in cell culture, purification, and quantification of infectious particles through viral plaque assay are described.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Virology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Pandey, P (Mrs Pratikshya Pandey)
UTAS Author:Karupiah, G (Associate Professor Guna Karupiah)
ID Code:131672
Year Published:2018
Deposited By:Medicine
Deposited On:2019-03-29
Last Modified:2019-04-18
Downloads:0

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