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Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini

Citation

Johnson, TK and Henstridge, MA and Herr, A and Moore, KA and Whisstock, JC and Warr, C, Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini, Nature Communications, 6 ISSN 2041-1723 (2015) [Refereed Article]


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Copyright Statement

Copyright 2015 Macmillan Publishers Limited. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/ncomms9759

Abstract

Patterning of the Drosophila embryonic termini is achieved by localized activation of the Torso receptor by the growth factor Trunk. Governing this event is the perforin-like protein Torso-like, which is localized to the extracellular space at the embryo poles and has long been proposed to control localized proteolytic activation of Trunk. However, a protease involved in terminal patterning remains to be identified, and the role of Torso-like remains unknown. Here we find that Trunk is cleaved intracellularly by Furin proteases. We further show that Trunk is secreted, and that levels of extracellular Trunk are greatly reduced in torso-like null mutants. On the basis of these and previous findings, we suggest that Torso-like functions to mediate secretion of Trunk, thus providing the mechanism for spatially restricted activation of Torso. Our data represent an alternative mechanism for the spatial control of receptor signalling, and define a different role for perforin-like proteins in eukaryotes. © 2015 Macmillan Publishers Limited. All rights reserved.

Item Details

Item Type:Refereed Article
Keywords:anatomy; data interpretation; embryo; eukaryote; fly; protein; secretion; Eukaryota; Drosophila protein; Fur1 protein, Drosophila; Fur2 protein, Drosophila; furin; Torso-like protein, Drosophila; trunk protein, Drosophila; animal; Drosophila; female
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Developmental genetics (incl. sex determination)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Warr, C (Professor Coral Warr)
ID Code:131333
Year Published:2015
Web of Science® Times Cited:22
Deposited By:Medicine
Deposited On:2019-03-13
Last Modified:2019-05-31
Downloads:12 View Download Statistics

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