eCite Digital Repository

A role for the Drosophila zinc transporter Zip88E in protecting against dietary zinc toxicity

Citation

Richards, CD and Warr, C and Burke, R, A role for the Drosophila zinc transporter Zip88E in protecting against dietary zinc toxicity, PLoS ONE, 12, (7) Article e0181237. ISSN 1932-6203 (2017) [Refereed Article]


Preview
PDF
2Mb
  

Copyright Statement

Copyright 2017 Richards et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0181237

Abstract

Zinc absorption in animals is thought to be regulated in a local, cell autonomous manner with intestinal cells responding to dietary zinc content. The Drosophila zinc transporter Zip88E shows strong sequence similarity to Zips 42C.1, 42C.2 and 89B as well as mammalian Zips 1, 2 and 3, suggesting that it may act in concert with the apically-localised Drosophila zinc uptake transporters to facilitate dietary zinc absorption by importing ions into the midgut enterocytes. However, the functional characterisation of Zip88E presented here indicates that Zip88E may instead play a role in detecting and responding to zinc toxicity. Larvae homozygous for a null Zip88E allele are viable yet display heightened sensitivity to elevated levels of dietary zinc. This decreased zinc tolerance is accompanied by an overall decrease in Metallothionein B transcription throughout the larval midgut. A Zip88E reporter gene is expressed only in the salivary glands, a handful of enteroendocrine cells at the boundary between the anterior and middle midgut regions, and in two parallel strips of sensory cell projections connecting to the larval ventral ganglion. Zip88E expression solely in this restricted subset of cells is sufficient to rescue the Zip88E mutant phenotype. Together, our data suggest that Zip88E may be functioning in a small subset of cells to detect excessive zinc levels and induce a systemic response to reduce dietary zinc absorption and hence protect against toxicity.

Item Details

Item Type:Refereed Article
Keywords:unclassified drug, zinc, zinc transporter, Zip88E protein, carrier protein, zinc, zinc-binding protein, adult, animal experiment, animal tissue, arthropod larva
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Warr, C (Professor Coral Warr)
ID Code:131314
Year Published:2017
Web of Science® Times Cited:5
Deposited By:Medicine
Deposited On:2019-03-13
Last Modified:2019-04-26
Downloads:10 View Download Statistics

Repository Staff Only: item control page