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Fully-automated in-syringe dispersive liquid-liquid microextraction for the determination of caffeine in coffee beverages
journal contribution
posted on 2023-05-19, 20:33 authored by Frizzarin, RM, Fernando Maya AlejandroFernando Maya Alejandro, Estela, JM, Cerda, VA novel fully-automated magnetic stirring-assisted lab-in-syringe analytical procedure has been developed for the fast and efficient dispersive liquid-liquid microextraction (DLLME) of caffeine in coffee beverages. The procedure is based on the microextraction of caffeine with a minute amount of dichloromethane, isolating caffeine from the sample matrix with no further sample pretreatment. Selection of the relevant extraction parameters such as the dispersive solvent, proportion of aqueous/organic phase, pH and flow rates have been carefully evaluated.
Caffeine quantification was linear from 2 to 75 mg L−1, with detection and quantification limits of 0.46 mg L−1 and 1.54 mg L−1, respectively. A coefficient of variation (n = 8; 5 mg L−1) of a 2.1% and a sampling rate of 16 h−1, were obtained. The procedure was satisfactorily applied to the determination of caffeine in brewed, instant and decaf coffee samples, being the results for the sample analysis validated using high-performance liquid chromatography.
History
Publication title
Food ChemistryVolume
212Pagination
759-767ISSN
0308-8146Department/School
School of Natural SciencesPublisher
Elsevier Sci LtdPlace of publication
The Boulevard, Langford Lane, Kidlington, Oxford, England, Oxon, Ox5 1GbRights statement
Copyright 2016 Elsevier LtdRepository Status
- Restricted