Moore et al 2002 Perkinsus.pdf (1.54 MB)
The development of a positive non-infectious control for the detection of Perkinsus using the Ray test
journal contribution
posted on 2023-05-19, 12:24 authored by Bradley MooreBradley Moore, Kleeman, SN, Lester, RJGTo establish a noncontagious control for the Ray thioglycollate test for the detection of Perkinsus in mollusks we evaluated nonviable stages of P. olseni for enlargement of hypnospores and blue/black iodine stain. Trophozoites made nonviable with formalin, irradiation or colchicine failed to swell in thioglycollate. They remained small and did not differentially stain in iodine. Trophozoites that had already developed into hypnospores in thioglycollate were rendered inactive by freezing, ethanol or formalin immersion. They retained their iodinophilic properties and thus could provide a partial control for the Ray Test.
History
Publication title
Journal of Shellfish ResearchVolume
21Pagination
871-873ISSN
0730-8000Department/School
Institute for Marine and Antarctic StudiesPublisher
Natl Shellfisheries AssocPlace of publication
C/O Dr. Sandra E. Shumway, Natural Science Division, Southampton College, Southampton, USA, Ny, 11968Rights statement
Copyright 2002 National Shellfish AssociationRepository Status
- Open