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Three nuclear genes for phylogenetic, SNP and population genetic studies of molluscs and other invertebrates
journal contribution
posted on 2023-05-19, 11:07 authored by Asta AudzijonyteAsta Audzijonyte, Vrijenhoek, RCThe study reports new primers capable of amplifying fragments from three nuclear protein-coding genes in a variety of deep-sea molluscs and annelids – adenine nucleotide translocase (Ant), calmodulin (Cal) and cyclophilin A (CycA). The Ant primers appear to be restricted to bivalve molluscs, whereas the Cal and CycA primers also amplified appropriate gene fragments from Lepetodrilus gastropod molluscs and Osedax polychaete worms. The amplified fragment of Cal contains an intron in the molluscs, but no intron was detected in the Ant and CycA fragments from any of the tested animals. DNA sequences generated by the three primer sets exhibited one to 15 single nucleotide polymorphism sites in deep-sea vesicomyid clams and Osedax boneworms. The observed levels of polymorphism indicate that the genes are likely to be useful in both population genetic and phylogenetic analyses of different invertebrate taxa.
History
Publication title
Molecular Ecology ResourcesVolume
10Pagination
200-204ISSN
1755-098XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
Wiley-Blackwell Publishing Ltd.Place of publication
United KingdomRights statement
Copyright 2009 Blackwell Publishing LtdRepository Status
- Restricted