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Acrylic acid surface-modified contact lens for the culture of limbal stem cells
journal contribution
posted on 2023-05-19, 10:46 authored by Zhang, H, Brown, KD, Lowe, SP, Guei-Sheung LiuGuei-Sheung Liu, Steele, D, Abberton, K, Daniell, MSurface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid:octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.
History
Publication title
Tissue Engineering. Part AVolume
20Issue
11-12Pagination
1593-1602ISSN
1937-3341Department/School
Menzies Institute for Medical ResearchPublisher
Mary Ann Liebert, Inc. PublishersPlace of publication
United StatesRights statement
Copyright 2014 Mary Ann Liebert, Inc.Repository Status
- Restricted