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Acrylic acid surface-modified contact lens for the culture of limbal stem cells


Zhang, H and Brown, KD and Lowe, SP and Liu, GS and Steele, D and Abberton, K and Daniell, M, Acrylic acid surface-modified contact lens for the culture of limbal stem cells, Tissue Engineering. Part A, 20, (11-12) pp. 1593-1602. ISSN 1937-3341 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Mary Ann Liebert, Inc.

DOI: doi:10.1089/ten.TEA.2013.0320


Surface treatment to a biomaterial surface has been shown to modify and help cell growth. Our aim was to determine the best surface-modified system for the treatment of limbal stem cell deficiency (LSCD), which would facilitate expansion of autologous limbal epithelial cells, while maintaining cultivated epithelial cells in a less differentiated state. Commercially available contact lenses (CLs) were variously surface modified by plasma polymerization with ratios of acrylic acid to octadiene tested at 100% acrylic acid, 50:50% acrylic acid:octadiene, and 100% octadiene to produce high-, mid-, and no-acid. X-ray photoelectron spectroscopy was used to analyze the chemical composition of the plasma polymer deposited layer. Limbal explants cultured on high acid-modified CLs outgrew more cells. Immunofluorescence and RT2-PCR array results indicated that a higher acrylic acid content can also help maintain progenitor cells during ex vivo expansion of epithelial cells. This study provides the first evidence for the ability of high acid-modified CLs to preserve the stemness and to be used as substrates for the culture of limbal cells in the treatment of LSCD.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biotechnology
Research Field:Gene and molecular therapy
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Liu, GS (Associate Professor Guei-Sheung Liu)
ID Code:120789
Year Published:2014
Web of Science® Times Cited:7
Deposited By:Menzies Institute for Medical Research
Deposited On:2017-08-30
Last Modified:2019-05-07

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