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Methanotrophic bacteria associated to rice roots: the cultivar effect assessed by T-RFLP and microarray analysis

journal contribution
posted on 2023-05-19, 05:20 authored by Luke, C, Bodrossy, L, Lupotto, E, Frenzel, P
Rice plants play a key role in regulating methane emissions from paddy fields by affecting both underlying processes: methane production and oxidation. Specific differences were reported for methane oxidation rates; however, studies on the bacterial communities involved are rare. Here, we analysed the methanotrophic community on the roots of 18 different rice cultivars by pmoA-based terminal restriction fragment length polymorphism (T-RFLP) and microarray analysis. Both techniques showed comparable and consistent results revealing a high diversity dominated by type II and type Ib methanotrophs. pmoA microarrays have been successfully used to study methane-oxidizing bacteria in various environments. However, the microarray's full potential resolving community structure has not been exploited yet. Here, we provide an example on how to include this information into multivariate statistics. The analysis revealed a rice cultivar effect on the methanotroph community composition that could be affiliated to the plant genotype. This effect became only significant by including the specific phylogenetic resolution provided by the microarray into the statistical analysis.

History

Publication title

Environmental Microbiology Reports

Issue

5

Pagination

518-525

ISSN

1758-2229

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Wiley-Blackwell Publishing Ltd.

Place of publication

Chichester, West Sussex PO19 8QG United Kingdom

Rights statement

Copyright 2011 Society for Applied Microbiology and Blackwell Publishing Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Rice

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