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Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis
journal contribution
posted on 2023-05-19, 02:33 authored by Thang, LY, See, HH, Joselito QuirinoMicelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
37Issue
9Pagination
1166-1169ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley - V C H Verlag GmbH & Co. KGaAPlace of publication
GermanyRights statement
Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted