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Reliability of Haemophilus influenzae biofilm measurement via static method, and determinants of in vitro biofilm production
journal contribution
posted on 2023-05-18, 23:37 authored by Obaid, NA, Stephen TristramStephen Tristram, Christian NarkowiczChristian Narkowicz, Glenn JacobsonGlenn JacobsonInformation is lacking regarding the precision of microtitre plate (MTP) assays used to measure biofilm. This study investigated the precision of an MTP assay to measure biofilm production by nontypeable Haemophilus influenzae (NTHi) and the effects of frozen storage and inoculation technique on biofilm production. The density of bacterial final growth was determined by absorbance after 18-20 h incubation, and biofilm production was then measured by absorbance after crystal violet staining. Biofilm formation was categorised as high and low for each strain. For the high biofilm producing strains of NTHi, interday reproducibility of NTHi biofilm formation measured using the MTP assay was excellent and met the acceptance criteria, but higher variability was observed in low biofilm producers. Method of inoculum preparation was a determinant of biofilm formation with inoculum prepared directly from solid media showing increased biofilm production for at least one of the high producing strains. In general, storage of NTHi cultures at -80 °C for up to 48 weeks did not have any major effect on their ability to produce biofilm.
History
Publication title
Canadian Journal of MicrobiologyVolume
62Issue
12Pagination
1013-1020ISSN
0008-4166Department/School
School of Pharmacy and PharmacologyPublisher
Natl Research Council CanadaPlace of publication
CanadaRights statement
Copyright remains with the author(s) or their institution(s).Repository Status
- Restricted