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Reliability of Haemophilus influenzae biofilm measurement via static method, and determinants of in vitro biofilm production


Obaid, NA and Tristram, S and Narkowicz, CK and Jacobson, GA, Reliability of Haemophilus influenzae biofilm measurement via static method, and determinants of in vitro biofilm production, Canadian Journal of Microbiology, 62, (12) pp. 1013-1020. ISSN 0008-4166 (2016) [Refereed Article]

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DOI: doi:10.1139/cjm-2016-0228


Information is lacking regarding the precision of microtitre plate (MTP) assays used to measure biofilm. This study investigated the precision of an MTP assay to measure biofilm production by nontypeable Haemophilus influenzae (NTHi) and the effects of frozen storage and inoculation technique on biofilm production. The density of bacterial final growth was determined by absorbance after 18-20 h incubation, and biofilm production was then measured by absorbance after crystal violet staining. Biofilm formation was categorised as high and low for each strain. For the high biofilm producing strains of NTHi, interday reproducibility of NTHi biofilm formation measured using the MTP assay was excellent and met the acceptance criteria, but higher variability was observed in low biofilm producers. Method of inoculum preparation was a determinant of biofilm formation with inoculum prepared directly from solid media showing increased biofilm production for at least one of the high producing strains. In general, storage of NTHi cultures at -80 C for up to 48 weeks did not have any major effect on their ability to produce biofilm.

Item Details

Item Type:Refereed Article
Keywords:biofilm formation, NTHi, nontypeable Haemophilus influenzae, reproducibility, bacterial isolate storage, microtitre plate assay
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Pharmaceutical sciences
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Obaid, NA (Miss Najlia Obaid)
UTAS Author:Tristram, S (Dr Stephen Tristram)
UTAS Author:Narkowicz, CK (Dr Christian Narkowicz)
UTAS Author:Jacobson, GA (Associate Professor Glenn Jacobson)
ID Code:112498
Year Published:2016
Deposited By:Pharmacy
Deposited On:2016-11-14
Last Modified:2017-11-02

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