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Amyloid β accumulation and inner retinal degenerative changes in Alzheimer's disease transgenic mouse
journal contribution
posted on 2023-05-18, 20:49 authored by Gupta, VK, Chitranshi, N, Gupta, VB, Golzan, M, Dheer, Y, Wall, RV, Georgevsky, D, Anna KingAnna King, James VickersJames Vickers, Chung, R, Graham, SThe APP-PS1ΔE9 mouse model of Alzheimer’s disease (AD) exhibits age dependent amyloid β (Aβ) plaque formation in their central nervous system due to high expression of mutated human APP and PSEN1 transgenes. Here we evaluated Aβ deposition and changes in soluble Aβ accumulation in the retinas of aged APP-PS1 mice using a combination of immunofluorescence, retinal flat mounts and western blotting techniques. Aβ accumulation in the retina has previously been shown to be associated with retinal ganglion cell apoptosis in animal models of glaucoma. This study investigated changes in the inner retinal function and structure in APP-PS1 mice using electrophysiology and histological approaches respectively. We report for the first time a significant decline in scotopic threshold response (STR) amplitudes which represents inner retinal function in transgenic animals compared to the wild type counterparts (p < 0.0001). Thinning of the retina particularly involving inner retinal layers and reduction in axonal density in the optic nerve was also observed. TUNEL staining was performed to examine neuronal apoptosis in the inner retina. Intraocular pressure (IOP) measurements showed that APP-PS1ΔE9 mice had a slightly elevated IOP, but the significance of this finding is not yet known. Together, these results substantiate previous observations and highlight that APP-PS1ΔE9 mice show evidence of molecular, functional and morphological degenerative changes in the inner retina.
History
Publication title
Neuroscience LettersVolume
623Pagination
52-56ISSN
0304-3940Department/School
Wicking Dementia Research Education CentrePublisher
Elsevier Sci Ireland LtdPlace of publication
IrelandRights statement
© 2016 ElsevierRepository Status
- Restricted