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Inhibition of platelet aggregation by vanilloid-like agents is not mediated by transient receptor potential vanilloid-1 channels or cannabinoid receptors

journal contribution
posted on 2023-05-18, 18:27 authored by Almaghrabi, S, Dominic GeraghtyDominic Geraghty, Kiran AhujaKiran Ahuja, Adams, M
Vanilloid-like agents, including capsaicin, N-arachidonoyl-dopamine and N-oleoyldopamine inhibit platelet aggregation, however little is known about the precise mechanism(s) of action. The authors have previously shown that blocking of the capsaicin receptor, transient receptor potential vanilloid-1 (TRPV1), does not interfere with capsaicin action during adenosine diphosphate (ADP)-induced aggregation. This research is extended to investigate the effect of these vanilloid-like-agents on platelet count, and to test whether the effect of these agents is mediated through TRPV1 and/or cannabinoid (CB1 and CB2) receptors in the presence of other agonists, including collagen and arachidonic acid. Incubation of platelets with each of the individual vanilloids, or with receptor antagonists of TRPV1 (SB452533), CB1 (AM251) and CB2 (AM630), for up to 2 h did not significantly affect the platelet count. Similarly, the effect of individual vanilloids on the inhibition of platelet aggregation was not significantly different in the presence of receptor agonists compared to control, irrespective of the agonist used, suggesting that the inhibitory effect of vanilloids on platelet aggregation is independent of TRPV1, CB1 and CB2 receptors. Further research on the antiplatelet activity of vanilloids should focus on mechanisms other than those associated with vanilloid receptors.

History

Publication title

Clinical and Experimental Pharmacology and Physiology

Volume

43

Issue

6

Pagination

606-611

ISSN

1440-1681

Department/School

School of Humanities

Publisher

Wiley

Place of publication

Hoboken, USA

Rights statement

Copyright 2016 John Wiley & Sons Australia, Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Diagnosis of human diseases and conditions

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