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Novel elements of the chondrocyte stress response identified using an in vitro model of mouse cartilage degradation

conference contribution
posted on 2023-05-24, 22:26 authored by Richard WilsonRichard Wilson, Golub, SB, Angelucci, C, Bateman, JF, Fosang, AJ
Proteolytic destruction of the cartilage extracellular matrix is a key event in joint pathologies such as osteoarthritis. It is becoming increasingly evident that cartilage degradation is propagated by pro-inflammatory mediators such as interleukin-1 (IL-1). However, the contribution of inflammation to chondrocyte dysfunction and cartilage degeneration in OA is not fully understood. Here we used label-free quantitative proteomics to evaluate the chondrocyte response to IL-1 within a native cartilage ECM, using mouse femoral heads cultured with and without IL-1. We analyzed both cartilage tissue proteome and soluble factors released into the media (secretome). In addition, we investigated the IL-1 response in wild-type cartilage and in TS5deltacat cartilage lacking active ADAMTS-5, a key enzyme involved in destruction of the aggrecan network. Our proteomics analysis was further validated using whole-genome mRNA expression profiling. LTQ-Orbitrap analysis identified 368 proteins and 728 proteins in the cartilage secretome and proteome, respectively (protein FDR < 0.01%). Bioinformatic annotation of IL-1 modulated proteins identified significant protein groups associated with ECM-receptor interactions, the inflammatory response, peptidase activity, oxidoreductase activity, stress response and endoplasmic reticulum (adj. p < 0.05). These proteins included proteases/inhibitors (Mmp12, Lgmn, Cstl1 and Cst3), matrix components (Chad, Kera and Crispld1) and ER stress proteins (Manf, Creld2) previously not associated with the cartilage IL-1 response. Results in wild-type and TS5deltacat cartilage were highly consistent overall, although several proteins, including Timp1 and Arg1, were differentially modulated between the genotypes at both protein and mRNA levels. Finally, proteins of interest (Vnn1 and Mmp12) were further investigated using immunohistochemistry.

History

Publication title

Proceedings of the 38th Annual Scientific Meeting of the Matrix Biology Society of Australia and New Zealand

Place of publication

Hobart, Tasmania, Australia

Event title

Proceedings of the 38th Annual Scientific Meeting of the Matrix Biology Society of Australia and New Zealand

Event Venue

Hobart, Tasmania, Australia

Date of Event (Start Date)

2015-11-01

Date of Event (End Date)

2015-11-04

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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    University Of Tasmania

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