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Mating compatibility and encystment characteristics of Alexandrium catenella dinoflagellate strains from Chilean southern fjords

conference contribution
posted on 2023-05-23, 10:21 authored by Mardones, JI, Christopher BolchChristopher Bolch, Varela, D, Untari, L, Gustaaf HallegraeffGustaaf Hallegraeff
Resting cysts play an important role in the origin and initiation of Alexandrium dinoflagellate blooms in different coastal environments. Alexandrium catenella causes major economic impacts for the Chilean shellfish and fish farm industries, but the environmental conditions that regulate the encystment timing and cyst production rate in the seasonally variable fjords are poorly understood. We investigated mating compatibility and genetic relationships among 10 Chilean strains of A. catenella Group l using rDNA-ITS and LSU-rDNA sequences. Strain crossing experiments in all possible pairwise combinations showed variable reproductive compatibility among strains with high compatibilities among northern (Los Lagos) and southern (Magallanes) but not central (Aysén) strains. However, these mating differences were not reflected in the identical LSU-rDNA and rDNA-ITS sequences observed among the strains. Encystment was synchronized in all pairwise crosses, with cyst production starting 26 days and terminating 45 days after inoculation. Cyst production was variable among crosses ranging from 7±1.3 to 316.9±20.5 cysts ml-1. The successful pairwise crosses performed with strains from the northern Chilean fjords do not explain the low concentrations of cysts detected in this area. Instead, these low cyst concentrations might be the result of local circulation patterns leading to a net flux offshore.

Funding

Australian Research Council

History

Publication title

Marine and Fresh-water Harmful Algae: Proceedings of the 16th International Conference on Harmful Algae

Editors

A Lincoln MacKenzie

Pagination

159-162

ISBN

978-87-990827-5-9

Department/School

Institute for Marine and Antarctic Studies

Publisher

Nelson, New Zealand

Place of publication

Cawthron Institute

Event title

16th International Conference on Harmful Algae

Event Venue

Wellington, New Zealand

Date of Event (Start Date)

2014-10-27

Date of Event (End Date)

2014-10-31

Rights statement

Copyright unknown

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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