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High sensitive matrix-free mass spectrometry analysis of peptides using silicon nanowires-based digital microfluidic devices
Citation
Lapierre, F and Piret, G and Drobecq, H and Melnyk, O and Coffinier, Y and Thomy, V and Boukherroub, R, High sensitive matrix-free mass spectrometry analysis of peptides using silicon nanowires-based digital microfluidic devices, Lab on a Chip, 11, (9) pp. 1620-1628. ISSN 1473-0197 (2011) [Refereed Article]
Copyright Statement
Copyright 2011 The Royal Society of Chemistry
Official URL: http://dx.doi.org/10.1039/c0lc00716a
Abstract
We present for the first time an electrowetting on dielectric (EWOD) microfluidic system coupled to
a surface-assisted laser desorption-ionization (SALDI) silicon nanowire-based interface for mass
spectrometry (MS) analysis of small biomolecules. Here, the transfer of analytes has been achieved on
specific locations on the SALDI interface followed by their subsequent mass spectrometry analysis
without the use of an organic matrix. To achieve this purpose, a device comprising a digital microfluidic
system and a patterned superhydrophobic/superhydrophilic silicon nanowire interface was developed.
The digital microfluidic system serves for the displacement of the droplets containing analytes, via an
electrowetting actuation, inside the superhydrophilic patterns. The nanostructured silicon interface acts
as an inorganic target for matrix-free laser desorption-ionization mass spectrometry analysis of the
dried analytes. The proposed device can be easily used to realize several basic operations of a Lab-on-
Chip such as analyte displacement and rinsing prior to MS analysis. We have demonstrated that the
analysis of low molecular weight compounds (700 m/z) can be achieved with a very high sensitivity
(down to 10 fmol uL-1).
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Environmental Sciences |
Research Group: | Environmental biotechnology |
Research Field: | Environmental biotechnology diagnostics (incl. biosensors) |
Objective Division: | Manufacturing |
Objective Group: | Instrumentation |
Objective Field: | Medical instruments |
UTAS Author: | Lapierre, F (Dr Florian Lapierre) |
ID Code: | 99996 |
Year Published: | 2011 |
Web of Science® Times Cited: | 66 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2015-04-22 |
Last Modified: | 2022-09-01 |
Downloads: | 0 |
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