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Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.)

Citation

Neuman, C and Hatje, E and Bowman, JP and Katouli, M, Characterisation of lactic acid bacteria isolated from the hindgut of farmed Tasmanian Atlantic salmon (Salmo salar L.), International Journal of Probiotics and Prebiotics, 9, (4) pp. 119-128. ISSN 1555-1431 (2014) [Refereed Article]


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Copyright 2014 by New Century Health Publishers, LLC

Official URL: http://www.newcenturyhealthpublishers.com/probioti...

Abstract

Between July 2011 and May 2012, 16 pooled faecal samples were collected on eight occasions (40 fish/ occasion) from Atlantic salmon fed two commercial diets in a farm in Tasmania, Australia. From a second Tasmanian farm, 12 pooled faecal samples were collected on three occasions (80 fish/ occasion) from salmon fed four trial diets. From these samples a total of 160 lactic acid bacteria (LAB) were isolated on MRS agar. Strains were initially typed using PhP-LB plates (PhPlate, AB) and divided into common types (CTs). Strains which belonged to the same CT and found on numerous sampling occasions (n = 73) were identified using 16S rRNA sequencing and tested for cytotoxins production, bacteriocins, adherence to Atlantic salmon kidney cells as well as competitively exclude 16 bacterial strains in vitro. These strains were also tested for their resistance against nine antibiotics commonly used in Aquaculture. The 73 LAB strains belonged to six species namely Enterococcus casseliflavus, E. faecalis, E. faecium, Pediococcus acidilactici, and Weisella hellenica of which, three different strains of W. hellenica and one P. acidilactici met most criteria including medium to high adherence and pathogen exclusion capability in vitro. The potential of these strains to serve as probiotic candidates is discussed.

Item Details

Item Type:Refereed Article
Keywords:salmon, aquaculture, probiotics, feeds, lactic acid bacteria
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Bowman, JP (Associate Professor John Bowman)
ID Code:99974
Year Published:2014
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-04-22
Last Modified:2015-05-07
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