eCite Digital Repository
The Xenopus Eomesodermin promoter and its concentration-dependent response to activin
Citation
Ryan, K and Garrett, N and Bourillot, P-Y and Stennard, FA and Gurdon, JB, The Xenopus Eomesodermin promoter and its concentration-dependent response to activin, Mechanisms of Development, 94 pp. 133-146. ISSN 0925-4773 (2000) [Refereed Article]
Copyright Statement
Copyright 2000 ELsevier Science Ireland Ltd.
Abstract
Eomesodermin is an essential early gene in Xenopus mesoderm formation and shows a morphogen-like response to activin. Here we defne the regions of the Eomesodermin promoter required for mesodermal expression and for concentration-dependent response to activin. We find an activin response element (ARE) located between 25.6 and 25.0 kb which contains two critical FAST2 binding sites. The ARE alone is necessary and sufficient for concentration-dependent response to activin. A 5.6 kb promoter recapitulates Eomes expression in normal
mesoderm cells. A repressor element extinguishes Eomes expression in the endoderm. We relate our results to mesoderm patterning in early Xenopus development and to a mechanism of morphogen gradient response.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | mesoderm induction, T-box transcription factor, embryogenesis |
| Research Division: | Biological Sciences |
| Research Group: | Genetics |
| Research Field: | Developmental Genetics (incl. Sex Determination) |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| Author: | Stennard, FA (Dr Fiona Stennard) |
| ID Code: | 99862 |
| Year Published: | 2000 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2015-04-13 |
| Last Modified: | 2015-05-05 |
| Downloads: | 0 |
Repository Staff Only: item control page