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Single cells can sense their position in a morphogen gradient

Citation

Gurdon, JB and Standley, H and Dyson, S and Butler, K and Langon, T and Ryan, K and Stennard, FA and Shimizu, K and Zorn, A, Single cells can sense their position in a morphogen gradient, Development (Cambridge), 126, (23) pp. 5309-17. ISSN 0950-1991 (1999) [Refereed Article]

Copyright Statement

Copyright 1999 The Company of Biologists Limited

Official URL: http://dev.biologists.org/content/126/23/5309.full...

Abstract

Xenopus blastula cells show a morphogen-like response to activin by expressing different genes according to the concentration of activin to which they are exposed. To understand how cells recognize their position in a concentration gradient, it is essential to know whether each cell responds individually to activin concentration. An alternative idea, proposed by previous work, is that cells need to interact with their neighbours to generate a concentration-related response. To distinguish between these ideas, we have cultured blastula cells under conditions which provide different degrees of contact with other cells, allowing nil to maximum communication with their neighbours. The cultures include cells attached to fibronectin and cells resting unattached on an agarose base. The cultures also include cells that have no contact with any cell except their clonal progeny, cells that have lateral contact to neighbouring cells, and cells that are completely enveloped by other cells in a reaggregate. We have used RNase protection and in situ hybridization to assay the expression of the activin-responsive Xenopus genes Xbra, Xgsc, Xeomes, Xapod, Xchordin, Mix1, Xlim1 and Cerberus. We find no difference in gene expression between cells attached to fibronectin and those unattached on agarose. Most importantly, we find that cells respond to activin in a concentration-related way irrespective of their degree of contact with other cells. Therefore interaction among cells is not required for the interpretation of morphogen concentration, at least in the case of the early genes studied here. We conclude that isolated blastula cells can sense and respond individually to activin by expressing genes in a concentration-dependent way.

Item Details

Item Type:Refereed Article
Keywords:morphogen gradient
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Stennard, FA (Dr Fiona Stennard)
ID Code:99858
Year Published:1999
Web of Science® Times Cited:55
Deposited By:Menzies Institute for Medical Research
Deposited On:2015-04-13
Last Modified:2015-05-04
Downloads:0

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