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Two distinct Staufen isoforms in Xenopus are vegetally localized during oogenesis
Citation
Allison, R and Czaplinski, K and Adegbenro, E and Stennard, FA and Houliston, E and Standart, N, Two distinct Staufen isoforms in Xenopus are vegetally localized during oogenesis, R N A: (Ribonucleic Acid), 10, (11) pp. 1751-63. ISSN 1355-8382 (2004) [Refereed Article]
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Copyright Statement
Copyright 2004 RNA Society
Abstract
Localization of mRNA is an important way of generating early asymmetries in the developing embryo. In Drosophila, Staufen is intimately involved in the localization of maternally inherited mRNAs critical for cell fate determination in the embryo. We show that double-stranded RNA-binding Staufen proteins are present in the oocytes of a vertebrate, Xenopus, and are localized to the vegetal cytoplasm, a region where important mRNAs including VegT and Vg1 mRNA become localized. We identified two Staufen isoforms named XStau1 and XStau2, where XStau1 was found to be the principal Staufen protein in oocytes, eggs, and embryos, the levels of both proteins peaking during mid-oogenesis. In adults, Xenopus Staufens are principally expressed in ovary and testis. XStau1 was detectable throughout the oocyte cytoplasm by immunofluorescence and was concentrated in the vegetal cortical region from stage II onward. It showed partial codistribution with subcortical endoplasmic reticulum (ER), raising the possibility that Staufen may anchor mRNAs to specific ER-rich domains. We further showed that XStau proteins are transiently phosphorylated by the MAPK pathway during meiotic maturation, a period during which RNAs such as Vg1 RNA are released from their tight localization at the vegetal cortex. These findings provide evidence that Staufen proteins are involved in targeting and/or anchoring of maternal determinants to the vegetal cortex of the oocyte in Xenopus. The Xenopus oocyte should thus provide a valuable system to dissect the role of Staufen proteins in RNA localization and vertebrate development.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | cell polarity |
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and Cell Biology |
| Research Field: | Biochemistry and Cell Biology not elsewhere classified |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Environmental Sciences |
| Author: | Stennard, FA (Dr Fiona Stennard) |
| ID Code: | 99857 |
| Year Published: | 2004 |
| Web of Science® Times Cited: | 48 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2015-04-13 |
| Last Modified: | 2015-05-05 |
| Downloads: | 0 |
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