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Conformational Stability andDNA Binding Specificity of the Cardiac T-Box Transcription Factor Tbx20
Citation
Macindoe, I and Glockner, L and Vukasin, P and Stennard, FA and Costa, MW and Harvey, RP and Mackay, JP and Sunde, M, Conformational Stability andDNA Binding Specificity of the Cardiac T-Box Transcription Factor Tbx20, Journal of Molecular Biology, 389 pp. 606-618. ISSN 0022-2836 (2009) [Refereed Article]
Copyright Statement
Copyright 2009 Elsevier Ltd.
DOI: doi:10.1016/j.jmb.2009.04.056
Abstract
The transcription factor Tbx20 acts within a hierarchy of T-box factors in lineage specification and morphogenesis in the mammalian heart and is mutated in congenital heart disease. T-box family members share a ∼20-kDa DNA-binding domain termed the T-box. The question of how highly homologous T-box proteins achieve differential transcriptional control in heart development, while apparently binding to the same DNA sequence, remains unresolved. Here we show that the optimal DNA recognition sequence for the T-box of Tbx20 corresponds to a T-half-site. Furthermore, we demonstrate using purified recombinant domains that distinct T-boxes show significant differences in the affinity and kinetics of binding and in conformational stability, with the T-box of Tbx20 displaying molten globule character. Our data highlight unique features of Tbx20 and suggest mechanistic ways in which cardiac T-box factors might interact synergistically
and/or competitively within the cardiac regulatory network
Item Details
Item Type: | Refereed Article |
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Keywords: | T-box transcrition factor |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Biochemistry and cell biology not elsewhere classified |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the environmental sciences |
UTAS Author: | Stennard, FA (Dr Fiona Stennard) |
ID Code: | 99852 |
Year Published: | 2009 |
Web of Science® Times Cited: | 22 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2015-04-13 |
Last Modified: | 2015-05-05 |
Downloads: | 0 |
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