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High peripheral blood Th17 percent associated with poor lung function in cystic fibrosis
Citation
Mulcahy, EM and Hudson, JB and Beggs, SA and Reid, DW and Roddam, LF and Cooley, MA, High peripheral blood Th17 percent associated with poor lung function in cystic fibrosis, PLoS One, 10, (3) Article e0120912. ISSN 1932-6203 (2015) [Refereed Article]
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Copyright Statement
Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1371/journal.pone.0120912
Abstract
People with cystic fibrosis (CF) have been reported to make lung T cell responses that are biased towards T helper (Th) 2 or Th17. We hypothesized that CF-related T cell regulatory defects could be detected by analyzing CD4+ lymphocyte subsets in peripheral blood. Peripheral blood mononuclear cells from 42 CF patients (6 months–53 years old) and 78 healthy controls (2–61 years old) were analyzed for Th1 (IFN-γ+), Th2 (IL-4+), Th17 (IL-17+), Treg (FOXP3+), IL-10+ and TGF-β+ CD4+ cells. We observed higher proportions of Treg, IL-10+ and TGF-β+ CD4+ cells in CF adults (≥ 18 years old), but not children/adolescents, compared with controls. Within the CF group, high TGF-β+% was associated with chronic Pseudomonas aeruginosa lung infection (p < 0.006). We observed no significant differences between control and CF groups in the proportions of Th1, Th2 or Th17 cells, and no association within the CF group of any subset with sex, CFTR genotype, or clinical exacerbation. However, high Th17% was strongly associated with poor lung function (FEV1 % predicted) (p = 0.0008), and this association was strongest when both lung function testing and blood sampling were performed within one week. Our results are consistent with reports of CF as a Th17 disease and suggest that peripheral blood Th17 levels may be a surrogate marker of lung function in CF.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | cystic fibrosis, T cell |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Medical microbiology |
| Research Field: | Medical bacteriology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Mulcahy, EM (Miss Emily Mulcahy) |
| UTAS Author: | Hudson, JB (Ms Jo Hudson) |
| UTAS Author: | Beggs, SA (Dr Sean Beggs) |
| UTAS Author: | Reid, DW (Dr David Reid) |
| UTAS Author: | Roddam, LF (Dr Louise Roddam) |
| UTAS Author: | Cooley, MA (Associate Professor Margaret Cooley) |
| ID Code: | 99791 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 18 |
| Deposited By: | Medicine |
| Deposited On: | 2015-04-09 |
| Last Modified: | 2017-11-07 |
| Downloads: | 242 View Download Statistics |
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