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Evidence for induction of humoral and cytotoxic immune responses against devil facial tumor disease cells in Tasmanian devils (Sarcophilus harrisii) immunized with killed cell preparations

Citation

Kreiss, A and Brown, GK and Tovar, C and Lyons, AB and Woods, GM, Evidence for induction of humoral and cytotoxic immune responses against devil facial tumor disease cells in Tasmanian devils (Sarcophilus harrisii) immunized with killed cell preparations, Vaccine, 33, (26) pp. 3016-3025. ISSN 0264-410X (2015) [Refereed Article]


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Licensed under Creative Commons license Attribution-NonCommercial-No Derivatives 4.0 International (CC BY-NC-ND 4.0)by-nc-nd/4.0/)

DOI: doi:10.1016/j.vaccine.2015.01.039

Abstract

Tasmanian devils (Sarcophilus harrisii) risk extinction from a contagious cancer, devil facial tumour disease (DFTD) in which the infectious agent is the tumor cell itself. Because devils are unable to produce an immune response against the tumor cells no devil has survived 'infection'. To promote an immune response we immunized healthy devils with killed DFTD tumor cells in the presence of adjuvants. Immune responses, including cytotoxicity and antibody production, were detected in five of the six devils. The incorporation of adjuvants that act via toll like receptors may provide additional signals to break 'immunological ignorance'. One of these devils was protected against a challenge with viable DFTD cells. This was a short-term protection as re-challenge one year later resulted in tumor growth. These results suggest that Tasmanian devils can generate immune responses against DFTD cells. With further optimization of immune stimulation it should be possible to protect Tasmanian devils against DFTD with an injectable vaccine.

Item Details

Item Type:Refereed Article
Keywords:Tasmanian devil, devil facial tumor disease (DFTD), vaccine, cellular immunology, transmissible cancer, marsupial, montanide ELISA, flow cytometry, veterinary immunology
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
UTAS Author:Kreiss, A (Dr Alexandre Kreiss)
UTAS Author:Brown, GK (Ms Gabriella Brown)
UTAS Author:Tovar, C (Dr Cesar Tovar Lopez)
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
UTAS Author:Woods, GM (Professor Gregory Woods)
ID Code:99458
Year Published:2015
Funding Support:Australian Research Council (LP0989727)
Web of Science® Times Cited:31
Deposited By:Menzies Institute for Medical Research
Deposited On:2015-03-24
Last Modified:2017-11-07
Downloads:154 View Download Statistics

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