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Micropatterned substrates made by polymer bilayer dewetting and collagen nanoscale assembly support endothelial cell adhesion

Citation

Thickett, SC and Moses, J and Gamble, JR and Neto, C, Micropatterned substrates made by polymer bilayer dewetting and collagen nanoscale assembly support endothelial cell adhesion, Soft Matter, 8, (39) pp. 9996-10007. ISSN 1744-683X (2012) [Refereed Article]

Copyright Statement

Copyright 2012 - The Royal Society of Chemistry

DOI: doi:10.1039/c2sm26557e

Abstract

The ability to control protein and cell positioning on a microscopic scale is crucial in many biomedical applications, such as tissue engineering and the development of biosensors. We demonstrate here that the assembly of collagen on patterned surfaces produced by the dewetting of metastable poly(N-vinylpyrrolidone) (PNVP) films on top of polystyrene films supports the adhesion and survival of a biologically relevant cell type, human endothelial cells. Micropatterning of Type 1 collagen was achieved on such substrates by exploiting the different protein affinity of the two polymers, the effect of treatment with an air plasma, and the control over the nanoscale assembly of collagen using different adsorption conditions. The simplicity of the dewetting approach, coupled with the ability to coat and pattern non-planar substrates, gives rise to possible applications in the coating of biological implants such as arterial stents.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Physical Chemistry (incl. Structural)
Research Field:Colloid and Surface Chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Thickett, SC (Dr Stuart Thickett)
ID Code:99419
Year Published:2012
Web of Science® Times Cited:14
Deposited By:Chemistry
Deposited On:2015-03-24
Last Modified:2017-10-24
Downloads:0

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