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Bio-functional, lanthanide-labeled polymer particles by seeded emulsion polymerization and their characterization by novel ICP-MS detection

Citation

Thickett, SC and Abdelrahman, AI and Ornatsky, O and Bandura, D and Baranov, V and Winnik, MA, Bio-functional, lanthanide-labeled polymer particles by seeded emulsion polymerization and their characterization by novel ICP-MS detection, Journal of Analytical Atomic Spectrometry, 25, (3) pp. 269-281. ISSN 0267-9477 (2010) [Refereed Article]

Copyright Statement

Copyright 2010 Royal Society of Chemistry

DOI: doi:10.1039/b916850h

Abstract

We present the synthesis and characterization of monodisperse, sub-micron poly(styrene) (PS) particles loaded with up to and including 107 lanthanide (Ln) ions per particle. These particles have been synthesized by seeded emulsion polymerization with a mixture of monomer and a pre-formed Ln complex, and analyzed on a particle-by-particle basis by a unique inductively coupled plasma mass cytometer. Seed particles were prepared by surfactant-free emulsion polymerization (SFEP) to obtain large particle sizes in aqueous media. Extensive surface acid functionality was introduced using the acid-functional initiator ACVA, either during seed latex synthesis or in the second stage of polymerization. The loading of particles with three different Ln ions (Eu, Tb, and Ho) has proven to be close to 100% efficient on an individual and combined basis. Covalent attachment of metal-tagged peptides and proteins such as Neutravidin to the particle surface was shown to be successful and the number of bound species can be readily determined. We believe these particles can serve as precursors for multiplexed, bead-based bio-assays utilizing mass cytometric detection.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Spectrometry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Thickett, SC (Dr Stuart Thickett)
ID Code:99404
Year Published:2010
Web of Science® Times Cited:17
Deposited By:Chemistry
Deposited On:2015-03-23
Last Modified:2015-08-11
Downloads:0

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