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Protein micropatterns by PEG grafting on dewetted PLGA films

Citation

Ghezzi, M and Thickett, SC and Telford, AM and Easton, CD and Meagher, L and Neto, C, Protein micropatterns by PEG grafting on dewetted PLGA films, Langmuir, 30, (39) pp. 11714-11722. ISSN 0743-7463 (2014) [Refereed Article]

Copyright Statement

2014 American Chemical Society

DOI: doi:10.1021/la5018592

Abstract

The ability to control protein and cell positioning on a microscopic scale is crucial in many biomedical applications, such as single cell studies. We have developed and investigated the grafting of poly(ethylene glycol) (PEG) brushes onto poly(d,l-lactide-co-glycolide) (PLGA) thin films, which can be micropatterned by exploiting their spontaneous dewetting on top of polystyrene (PS) films. Dense PEG brushes with excellent protein repellence were achieved on PLGA by using cloud point grafting conditions, and selective adsorption of proteins on the micropatterned substrates was achieved by exploiting the different affinity protein adsorption onto the PEG brushes and the PS holes. PEG-grafted PLGA films showed better resistance against spontaneous degradation in buffer than bare PLGA films, due to passivation by the thin PEG coating. The simplicity of dewetting and subsequent grafting approaches, coupled with the ability to coat and pattern nonplanar substrates give rise to possible applications of PEG-grafted PLGA films in single cell studies and cell cultures for tissue engineering.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Physical Chemistry (incl. Structural)
Research Field:Colloid and Surface Chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Thickett, SC (Dr Stuart Thickett)
ID Code:99396
Year Published:2014
Web of Science® Times Cited:7
Deposited By:Chemistry
Deposited On:2015-03-23
Last Modified:2017-10-24
Downloads:0

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