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Metal-containing polystyrene beads as standards for mass cytometry
Citation
Abdelrahman, AI and Ornatsky, O and Bandura, D and Baranov, V and Kinach, R and Dai, S and Thickett, SC and Tanner, S and Winnik, MA, Metal-containing polystyrene beads as standards for mass cytometry, Journal of Analytical Atomic Spectrometry, 25 pp. 260-268. ISSN 0267-9477 (2010) [Refereed Article]
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Copyright Statement
Copyright 2010 The Royal Society of Chemistry
DOI: doi:10.1039/B921770C
Abstract
We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.
Item Details
Item Type: | Refereed Article |
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Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Analytical spectrometry |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Thickett, SC (Dr Stuart Thickett) |
ID Code: | 99328 |
Year Published: | 2010 |
Web of Science® Times Cited: | 35 |
Deposited By: | Chemistry |
Deposited On: | 2015-03-19 |
Last Modified: | 2015-04-14 |
Downloads: | 460 View Download Statistics |
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