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Metal-containing polystyrene beads as standards for mass cytometry

Citation

Abdelrahman, AI and Ornatsky, O and Bandura, D and Baranov, V and Kinach, R and Dai, S and Thickett, SC and Tanner, S and Winnik, MA, Metal-containing polystyrene beads as standards for mass cytometry, Journal of Analytical Atomic Spectrometry, 25 pp. 260-268. ISSN 0267-9477 (2010) [Refereed Article]


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Copyright Statement

Copyright 2010 The Royal Society of Chemistry

DOI: doi:10.1039/B921770C

Abstract

We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Spectrometry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Thickett, SC (Dr Stuart Thickett)
ID Code:99328
Year Published:2010
Web of Science® Times Cited:19
Deposited By:Chemistry
Deposited On:2015-03-19
Last Modified:2015-04-14
Downloads:329 View Download Statistics

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