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Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex

journal contribution
posted on 2023-05-18, 08:26 authored by Hooks, BM, John LinJohn Lin, Guo, C, Svoboda, K
Cortical cells integrate synaptic input from multiple sources, but how these different inputs are distributed across individual neurons is largely unknown. Differences in input might account for diverse responses in neighboring neurons during behavior. We present a strategy for comparing the strengths of multiple types of input onto the same neuron. We developed methods for independent dualchannel photostimulation of synaptic inputs using ChR2 together with ReaChR, a red-shifted channelrhodopsin. We used dual-channel photostimulation to probe convergence of sensory information in the mouse primary motor cortex. Input from somatosensory cortex and thalamus converges in individual neurons. Similarly, inputs from distinct somatotopic regions of the somatosensory cortex are integrated at the level of single motor cortex neurons. We next developed a ReaChR transgenic mouse under the control of both Flp- and Cre-recombinases that is an effective tool for circuit mapping. Our approach to dual-channel photostimulation enables quantitative comparison of the strengths of multiple pathways across all length scales of the brain.

History

Publication title

Journal of Neuroscience

Volume

35

Issue

10

Pagination

4418 - 4426

ISSN

0270-6474

Department/School

Tasmanian School of Medicine

Publisher

Soc Neuroscience

Place of publication

11 Dupont Circle, Nw, Ste 500, Washington, USA, Dc, 20036

Rights statement

Copyright © 2015 the authors

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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