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Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex
Citation
Hooks, BM and Lin, JY and Guo, C and Svoboda, K, Dual-channel circuit mapping reveals sensorimotor convergence in the primary motor cortex, Journal of Neuroscience, 35, (10) pp. 4418 - 4426. ISSN 0270-6474 (2015) [Refereed Article]
Copyright Statement
Copyright © 2015 the authors
DOI: doi:10.1523/JNEUROSCI.3741-14.2015
Abstract
Cortical cells integrate synaptic input from multiple sources, but how these different inputs are distributed across individual neurons is largely unknown. Differences in input might account for diverse responses in neighboring neurons during behavior. We present a
strategy for comparing the strengths of multiple types of input onto the same neuron. We developed methods for independent dualchannel photostimulation of synaptic inputs using ChR2 together with ReaChR, a red-shifted channelrhodopsin. We used dual-channel
photostimulation to probe convergence of sensory information in the mouse primary motor cortex. Input from somatosensory cortex and thalamus converges in individual neurons. Similarly, inputs from distinct somatotopic regions of the somatosensory cortex are integrated at the level of single motor cortex neurons. We next developed a ReaChR transgenic mouse under the control of both Flp- and Cre-recombinases that is an effective tool for circuit mapping. Our approach to dual-channel photostimulation enables quantitative comparison of the strengths of multiple pathways across all length scales of the brain.
Item Details
Item Type: | Refereed Article |
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Keywords: | circuit mapping, dual-channel stimulation, motor cortex, optogenetics, ReaChR, somatosensory cortex, optogenetics, neuroscience |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Neurosciences |
Research Field: | Central nervous system |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Lin, JY (Dr John Lin) |
ID Code: | 99101 |
Year Published: | 2015 |
Web of Science® Times Cited: | 42 |
Deposited By: | Medicine |
Deposited On: | 2015-03-13 |
Last Modified: | 2017-11-06 |
Downloads: | 0 |
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