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Expression of ephrin-A2 in the superior colliculus and EphA5 in the retina following optic nerve section in adult rat

Citation

Rodger, J and Lindsey, KA and Leaver, SG and King, CE and Dunlop, SA and Beazley, LD, Expression of ephrin-A2 in the superior colliculus and EphA5 in the retina following optic nerve section in adult rat, European Journal of Neuroscience, 14, (12) pp. 1929-1936. ISSN 0953-816X (2001) [Refereed Article]

Copyright Statement

Copyright 2001 Federation of European Neuroscience Societies.

DOI: doi:10.1046/j.0953-816x.2001.01822.x

Abstract

The vertebrate retina projects topographically to visual brain centres. In the developing visual system, gradients of ephrins and Eph receptors play a role in defining topography. At maturity, ephrins but not Ephs are downregulated. Here we show that optic nerve section in adult rat differentially regulates the expression of ephrin-A2 in the superior colliculus (SC) and of EphA5 in the retina. Expression was quantified immunohistochemically; ephrin-A2 levels were also estimated by semiquantitative reverse transcriptase polymerase chain reaction. In the normal SC, ephrin-A2 was expressed at low levels. At 1 month, levels of protein and of mRNA were upregulated across the contralateral SC giving rise to an increasing rostro-caudal gradient. At 6 months, levels had fallen but a gradient remained. In the retina of normal animals, EphA5 was expressed as an increasing naso-temporal gradient. By 1 month, expression was decreased in far temporal retina, resulting in a uniform expression across the naso-temporal axis. We suggest that denervation-induced plastic changes within the SC modify expression of these molecules.

Item Details

Item Type:Refereed Article
Keywords:Eph, ephrin, optic nerve, superior colliculus, topography
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:King, CE (Dr Carolyn King)
ID Code:99045
Year Published:2001
Web of Science® Times Cited:44
Deposited By:Health Sciences B
Deposited On:2015-03-11
Last Modified:2015-04-15
Downloads:0

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