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EphA/ephrin-A interactions during optic nerve regeneration: restoration of topography and regulation of ephrin-A2 expression

Citation

Rodger, J and Vitale, PN and Tee, LBG and King, CE and Bartlett, CA and Fall, A and Brennan, C and O'Shea, JE and Dunlop, SA and Beazley, LD, EphA/ephrin-A interactions during optic nerve regeneration: restoration of topography and regulation of ephrin-A2 expression, Molecular and Cellular Neurosciences, 25, (1) pp. 56-68. ISSN 1044-7431 (2004) [Refereed Article]

Copyright Statement

Copyright 2003 Elsevier Inc.

DOI: doi:10.1016/j.mcn.2003.09.010

Abstract

During visual system development, interactions between Eph tyrosine kinase receptors and their ligands, the ephrins, guide retinal ganglion cell (RGC) axons to their topographic targets in the optic tectum. Here we show that Eph/ephrin interactions are also involved in restoring topography during RGC axon regeneration in goldfish. Following optic nerve crush, EphA/ephrin-A interactions were blocked by intracranial injections of recombinant Eph receptor (EphA3-AP) or phospho-inositol phospholipase-C. Topographic errors with multiple inputs to some tectal loci were detected electrophysiologically and increased projections to caudal tectum demonstrated by RT-97 immunohistochemistry. In EphA3-AP-injected fish, ephrin-A2-expressing cells in the retino-recipient tectal layers were reduced in number compared to controls and their distribution was no longer graded. The findings, supported by in vitro studies, implicate EphA/ephrin-A interactions in restoring precise topography and in regulating ephrin-A2 expression during regeneration.

Item Details

Item Type:Refereed Article
Keywords:Eph, ephrin, optic nerve, regeneration, topography
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Nervous System and Disorders
Author:King, CE (Dr Carolyn King)
ID Code:99042
Year Published:2004
Web of Science® Times Cited:35
Deposited By:Health Sciences B
Deposited On:2015-03-11
Last Modified:2015-05-05
Downloads:0

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