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Pyrosequencing reveals transient cystic fibrosis lung microbiome changes with intravenous antibiotics

Citation

Smith, DJ and Badrick, AC and Zakrewski, M and Krause, L and Bell, SC and Anderson, GJ and Reid, DW, Pyrosequencing reveals transient cystic fibrosis lung microbiome changes with intravenous antibiotics, The European Respiratory Journal, 44, (4) pp. 922-930. ISSN 0903-1936 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 European Respiratory Society

DOI: doi:10.1183/09031936.00203013

Abstract

Chronic airway infection in adults with cystic fibrosis (CF) is polymicrobial and the impact of intravenous antibiotics on the bacterial community composition is poorly understood. We employed culture-independent molecular techniques to explore the early effects of i.v. antibiotics on the CF airway microbiome.

DNA was extracted from sputum samples collected from adult subjects with CF at three time-points (before starting treatment, and at day 3 and day 810 of i.v. antibiotics) during treatment of an infective pulmonary exacerbation. Microbial community profiles were derived through analysis of bacterial-derived 16S ribosomal RNA by pyrosequencing and changes over time were compared.

59 sputum samples were collected during 24 pulmonary exacerbations from 23 subjects. Between treatment onset and day 3 there was a significant reduction in the relative abundance of Pseudomonas and increased microbial diversity. By day 810, bacterial community composition was similar to pre-treatment. Changes in community composition did not predict improvements in lung function.

The relative abundance of Pseudomonas falls rapidly in subjects with CF receiving i.v. antibiotic treatment for a pulmonary exacerbation and is accompanied by an increase in overall microbial diversity. However, this effect is not maintained beyond the first week of treatment.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Reid, DW (Dr David Reid)
ID Code:98809
Year Published:2014
Web of Science® Times Cited:27
Deposited By:Medicine (Discipline)
Deposited On:2015-03-03
Last Modified:2015-04-22
Downloads:0

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