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Electrokinetics for sample preparation of biological molecules in biological samples using microfluidic systems


Shallan, AI and Guijt, RM and Breadmore, MC, Electrokinetics for sample preparation of biological molecules in biological samples using microfluidic systems, Bioanalysis, 6, (14) pp. 1961-1974. ISSN 1757-6180 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Future Science Ltd.

DOI: doi:10.4155/BIO.14.140


Sample preparation is the first part of every analytical method, but is often considered only after the optimization of the method. It is traditionally performed using a range of techniques requiring extensive manual handling, with solid-phase extraction, liquid–liquid extraction, protein precipitation and ultracentrfiguation, among others, being used depending on the targets and the application. In this article, we will focus on alternatives based on electrokinetics for applications including sample clean-up, concentration and derivatization of large biological molecules (DNA, peptides and proteins) of diagnostic importance, as well as small molecules as a tool for therapeutic drug monitoring. This article describes these approaches in terms of mechanisms, applicability and potential to be integrated into a lab-on-a-chip device for directly processing biological samples. Examples dealing with treated or clean samples have been excluded except where they show exceptionally high value.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Shallan, AI (Ms Aliaa Shallan)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:98745
Year Published:2014
Funding Support:Australian Research Council (FT130100101)
Web of Science® Times Cited:11
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-27
Last Modified:2017-10-28

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