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Evaluation of potential cationic probes for the detection of proline and betaine
journal contribution
posted on 2023-05-18, 07:55 authored by Kalsoom, U, Michael BreadmoreMichael Breadmore, Rosanne Guijt, Boyce, MCOsmoregulants are the substances that help plants to tolerate environmental extremes such as salinity and drought. Proline and betaine are two of the most commonly studied osmoregulants. An indirect UV CE method has been developed for simultaneous determination of these osmoregulants. A variety of reported probes and compounds were examined as potential probes for the indirect detection of proline and betaine. Mobility and UV-absorption properties highlighted sulfanilamide as a potential probe for indirect analysis of proline and betaine. Using 5 mM sulfanilamide at pH 2.2 with UV detection at 254 nm, proline and betaine were separated in less than 15 min. The LODs for proline and betaine were 11.6 and 28.3 μM, respectively. The developed method was successfully applied to quantification of these two osmoregulants in spinach and beetroot samples.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
35Issue
23Pagination
3379-3386ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
PO Box 10 11 61, Weinheim, Germany, D-69451Rights statement
Copyright 2014 Wiley-VCH Verlag GmbH & Co.Repository Status
- Restricted