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Evaluation of potential cationic probes for the detection of proline and betaine


Kalsoom, U and Breadmore, MC and Guijt, RM and Boyce, MC, Evaluation of potential cationic probes for the detection of proline and betaine, Electrophoresis, 35, (23) pp. 3379-3386. ISSN 0173-0835 (2014) [Refereed Article]

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Copyright 2014 Wiley-VCH Verlag GmbH & Co.

DOI: doi:10.1002/elps.201400303


Osmoregulants are the substances that help plants to tolerate environmental extremes such as salinity and drought. Proline and betaine are two of the most commonly studied osmoregulants. An indirect UV CE method has been developed for simultaneous determination of these osmoregulants. A variety of reported probes and compounds were examined as potential probes for the indirect detection of proline and betaine. Mobility and UV-absorption properties highlighted sulfanilamide as a potential probe for indirect analysis of proline and betaine. Using 5 mM sulfanilamide at pH 2.2 with UV detection at 254 nm, proline and betaine were separated in less than 15 min. The LODs for proline and betaine were 11.6 and 28.3 μM, respectively. The developed method was successfully applied to quantification of these two osmoregulants in spinach and beetroot samples.

Item Details

Item Type:Refereed Article
Keywords:electrophoresis, betaine, cationic probes, indirect detection, osmoregulants, proline
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:98742
Year Published:2014
Funding Support:Australian Research Council (FT130100101)
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-27
Last Modified:2017-10-28

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