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Growth hormone transgenesis and polyploidy increase metabolic rate, alter the cardiorespiratory response and influence HSP expression in response to acute hypoxia in Atlantic salmon (Salmo salar) yolk-sac alevins
Citation
Polymeropoulos, ET and Plouffe, D and LeBlanc, S and Elliot, NG and Currie, S and Frappell, PB, Growth hormone transgenesis and polyploidy increase metabolic rate, alter the cardiorespiratory response and influence HSP expression in response to acute hypoxia in Atlantic salmon (Salmo salar) yolk-sac alevins, Journal of Experimental Biology, 217, (13) pp. 2268-2276. ISSN 0022-0949 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 The Company of Biologists Ltd
Abstract
Growth hormone (GH)-transgenic Atlantic salmon display accelerated
growth rates compared with non-transgenics. GH-transgenic fish also
display cardiorespiratory and metabolic modifications that accompany
the increased growth rate. An elevated routine metabolic rate has
been described for pre- and post-smolt GH-transgenic salmon that
also display improvements in oxygen delivery to support the
increased aerobic demand. The early ontogenic effects of GH
transgenesis on the respiratory and cellular physiology of fish,
especially during adverse environmental conditions, and the effect of
polyploidy are unclear. Here, we investigated the effects of GH
transgenesis and polyploidy on metabolic, heart and ventilation rates
and heat shock protein (HSP) levels after exposure to acute hypoxia
in post-hatch Atlantic salmon yolk-sac alevins. Metabolic rate
decreased with decreasing partial pressures of oxygen in all
genotypes. In normoxia, triploid transgenics displayed the highest
mass-specific metabolic rates in comparison to diploid transgenics
and non-transgenic triploids, which, in contrast, had higher rates than
diploid non-transgenics. In hypoxia, we observed a lower massspecific
metabolic rate in diploid non-transgenics compared with all
other genotypes. However, no evidence for improved O2 uptake
through heart or ventilation rate was found. Heart rate decreased in
diploid non-transgenics while ventilation rate decreased in both
diploid non-transgenics and triploid transgenics in severe hypoxia.
Regardless of genotype or treatment, inducible HSP70 was not
expressed in alevins. Following hypoxia, the constitutive isoform of
HSP70, HSC70, decreased in transgenics and HSP90 expression
decreased in all genotypes. These data suggest that physiological
changes through GH transgenesis and polyploidy are manifested
during early ontogeny in Atlantic salmon.
Item Details
Item Type: | Refereed Article |
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Keywords: | Salmo salar, hypoxia, growth hormone transgenesis, polyploidy, metabolic rate, oxygen transport, heat shock proteins |
Research Division: | Biological Sciences |
Research Group: | Zoology |
Research Field: | Animal physiological ecology |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Aquaculture fin fish (excl. tuna) |
UTAS Author: | Polymeropoulos, ET (Dr Elias Polymeropoulos) |
UTAS Author: | Frappell, PB (Professor Peter Frappell) |
ID Code: | 98698 |
Year Published: | 2014 |
Web of Science® Times Cited: | 6 |
Deposited By: | Sustainable Marine Research Collaboration |
Deposited On: | 2015-02-25 |
Last Modified: | 2018-03-18 |
Downloads: | 0 |
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