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Growth hormone transgenesis and polyploidy increase metabolic rate, alter the cardiorespiratory response and influence HSP expression in response to acute hypoxia in Atlantic salmon (Salmo salar) yolk-sac alevins

Citation

Polymeropoulos, ET and Plouffe, D and LeBlanc, S and Elliot, NG and Currie, S and Frappell, PB, Growth hormone transgenesis and polyploidy increase metabolic rate, alter the cardiorespiratory response and influence HSP expression in response to acute hypoxia in Atlantic salmon (Salmo salar) yolk-sac alevins, Journal of Experimental Biology, 217, (13) pp. 2268-2276. ISSN 0022-0949 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 The Company of Biologists Ltd

DOI: doi:10.1242/jeb.098913

Abstract

Growth hormone (GH)-transgenic Atlantic salmon display accelerated growth rates compared with non-transgenics. GH-transgenic fish also display cardiorespiratory and metabolic modifications that accompany the increased growth rate. An elevated routine metabolic rate has been described for pre- and post-smolt GH-transgenic salmon that also display improvements in oxygen delivery to support the increased aerobic demand. The early ontogenic effects of GH transgenesis on the respiratory and cellular physiology of fish, especially during adverse environmental conditions, and the effect of polyploidy are unclear. Here, we investigated the effects of GH transgenesis and polyploidy on metabolic, heart and ventilation rates and heat shock protein (HSP) levels after exposure to acute hypoxia in post-hatch Atlantic salmon yolk-sac alevins. Metabolic rate decreased with decreasing partial pressures of oxygen in all genotypes. In normoxia, triploid transgenics displayed the highest mass-specific metabolic rates in comparison to diploid transgenics and non-transgenic triploids, which, in contrast, had higher rates than diploid non-transgenics. In hypoxia, we observed a lower massspecific metabolic rate in diploid non-transgenics compared with all other genotypes. However, no evidence for improved O2 uptake through heart or ventilation rate was found. Heart rate decreased in diploid non-transgenics while ventilation rate decreased in both diploid non-transgenics and triploid transgenics in severe hypoxia. Regardless of genotype or treatment, inducible HSP70 was not expressed in alevins. Following hypoxia, the constitutive isoform of HSP70, HSC70, decreased in transgenics and HSP90 expression decreased in all genotypes. These data suggest that physiological changes through GH transgenesis and polyploidy are manifested during early ontogeny in Atlantic salmon.

Item Details

Item Type:Refereed Article
Keywords:Salmo salar, hypoxia, growth hormone transgenesis, polyploidy, metabolic rate, oxygen transport, heat shock proteins
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Physiological Ecology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Polymeropoulos, ET (Dr Elias Polymeropoulos)
Author:Frappell, PB (Professor Peter Frappell)
ID Code:98698
Year Published:2014
Web of Science® Times Cited:3
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2015-02-25
Last Modified:2017-11-01
Downloads:0

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