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Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia

Citation

Yuskianti, V and Glen, M and Puspitasari, D and Francis, A and Rimbawanto, A and Gafur, A and Indrayadi, H and Mohammed, CL, Species-specific PCR for rapid identification of Ganoderma philippii and Ganoderma mastoporum from Acacia mangium and Eucalyptus pellita plantations in Indonesia, Forest Pathology, 44, (6) pp. 477-485. ISSN 1439-0329 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Blackwell Verlag GmbH

DOI: doi:10.1111/efp.12144

Abstract

DNA sequence variation in the rDNA internal transcribed spacers (ITS) was exploited to design species-specific primers for Ganoderma philippii and Ganoderma mastoporum, two fungi associated with red root rot in Acacia mangium and other perennial crops. Specificity was verified against five other Ganoderma species, 34 other basidiomycete species and two ascomycete species. The PCR tests assisted in the identification of 822 fungal isolates obtained from A. mangium and Eucalyptus pellita plantations in Indonesia. These tests have provided a high level of confidence in the identification of the major root rot pathogens, information that can be used to support the development of management options for their control in A. mangium plantations.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Forestry Sciences
Research Field:Forestry Pests, Health and Diseases
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood Plantations
Author:Glen, M (Dr Morag Glen)
Author:Francis, A (Mr Anthony Francis)
Author:Mohammed, CL (Associate Professor Caroline Mohammed)
ID Code:98636
Year Published:2014
Web of Science® Times Cited:9
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-02-23
Last Modified:2017-11-09
Downloads:0

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