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A trade off between separation, detection and sustainability in liquid chromatographic fingerprinting


Funari, CS and Carneiro, RL and Cavalheiro, AJ and Hilder, EF, A trade off between separation, detection and sustainability in liquid chromatographic fingerprinting, Journal of Chromatography A, 1354 pp. 34-42. ISSN 0021-9673 (2014) [Refereed Article]

Copyright Statement

2014 Published by Elsevier B.V.

DOI: doi:10.1016/j.chroma.2014.05.018


It is now recognized that analytical chemistry must also be a target for green principles, in particular chromatographic methods which typically use relatively large volumes of hazardous organic solvents. More generally, high performance liquid chromatography (HPLC) is employed routinely for quality control of complex mixtures in various industries. Acetonitrile and methanol are the most commonly used organic solvents in HPLC, but they generate an impact on the environment and can have a negative effect on the health of analysts. Ethanol offers an exciting alternative as a less toxic, biodegradable solvent for HPLC. In this work we demonstrate that replacement of acetonitrile with ethanol as the organic modifier for HPLC can be achieved without significantly compromising analytical performance. This general approach is demonstrated through the specific example analysis of a complex plant extract. A benchmark method employing acetonitrile for the analysis of Bidens pilosa extract was statistically optimized using the Green Chromatographic Fingerprinting Response (GCFR) which includes factors relating to separation performance and environmental parameters. Methods employing ethanol at 30 and 80C were developed and compared with the reference method regarding their performance of separation (GCFR) as well as by a new metric, Comprehensive Metric to Compare Liquid Chromatography Methods (CM). The fingerprint with ethanol at 80C was similar to or better than that with MeCN according to GCFR and CM. This demonstrates that temperature may be used to replace harmful solvents with greener ones in HPLC, including for solvents with significantly different physiochemical properties and without loss in separation performance. This work offers a general approach for the chromatographic analysis of complex samples without compromising green analytical chemistry principles.

Item Details

Item Type:Refereed Article
Keywords:green chromatography, green solvents, solvent replacement, high temperature liquid chromatography, green metabolite profiling
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Funari, CS (Dr Cristiano Funari)
UTAS Author:Hilder, EF (Professor Emily Hilder)
ID Code:98606
Year Published:2014
Web of Science® Times Cited:15
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-20
Last Modified:2018-03-14

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