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The development of the in situ modification of 1st generation analytical scale silica monoliths
journal contribution
posted on 2023-05-18, 07:43 authored by Soliven, A, Dennis, GR, Emily HilderEmily Hilder, Shalliker, RA, Stevenson, PGAnalytical scale silica monoliths are commercially limited to three column selectivities (bare silica, C8 and C18). An in situ modification is reported in detail to overcome this barrier and allow for any functionality of choice to be bonded to the silica surface of the monolithic stationary phase support. The modification method was conducted on a commercial bare silica column to bond the C18 moiety to the silica surface through a silylation reaction. The C18 type of stationary phase was chosen, as this is the most commonly bonded functionality for the majority of stationary phases used for high-performance liquid chromatography (HPLC) separations. The C18-modified monolith’s performance was compared to a commercial C18 monolithic and a particle packed column of the same analytical scale column dimensions (100 × 4.6 mm). The modified C18 monolith proved to be of high quality with an efficiency of 73,267 N m−1, fast analysis times (operated at flow rates up to 3 mL min−1 using a conventional 400 bar HPLC system) and improved resolution of a set of polar and non-polar substituted aromatics in comparison to a commercial C18 monolith.
History
Publication title
ChromatographiaVolume
77Issue
9-10Pagination
663-671ISSN
0009-5893Department/School
School of Natural SciencesPublisher
ViewegPlace of publication
Abraham-Lincoln-Strabe 46, Postfach 15 47, Wiesbaden, Germany, D-65005Rights statement
© Springer-Verlag Berlin Heidelberg 2014Repository Status
- Restricted