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The development of the in situ modification of 1st generation analytical scale silica monoliths

Citation

Soliven, A and Dennis, GR and Hilder, EF and Shalliker, RA and Stevenson, PG, The development of the in situ modification of 1st generation analytical scale silica monoliths, Chromatographia, 77, (9-10) pp. 663-671. ISSN 0009-5893 (2014) [Refereed Article]

Copyright Statement

© Springer-Verlag Berlin Heidelberg 2014

DOI: doi:10.1007/s10337-014-2667-z

Abstract

Analytical scale silica monoliths are commercially limited to three column selectivities (bare silica, C8 and C18). An in situ modification is reported in detail to overcome this barrier and allow for any functionality of choice to be bonded to the silica surface of the monolithic stationary phase support. The modification method was conducted on a commercial bare silica column to bond the C18 moiety to the silica surface through a silylation reaction. The C18 type of stationary phase was chosen, as this is the most commonly bonded functionality for the majority of stationary phases used for high-performance liquid chromatography (HPLC) separations. The C18-modified monolith’s performance was compared to a commercial C18 monolithic and a particle packed column of the same analytical scale column dimensions (100 × 4.6 mm). The modified C18 monolith proved to be of high quality with an efficiency of 73,267 N m−1, fast analysis times (operated at flow rates up to 3 mL min−1 using a conventional 400 bar HPLC system) and improved resolution of a set of polar and non-polar substituted aromatics in comparison to a commercial C18 monolith.

Item Details

Item Type:Refereed Article
Keywords:column liquid chromatography, stationary phase modification, in situ modification, bonded stationary phases, modified monolith, analytical silica monolith
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Hilder, EF (Professor Emily Hilder)
ID Code:98604
Year Published:2014
Web of Science® Times Cited:10
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-20
Last Modified:2017-10-28
Downloads:0

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