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Green chromatographic fingerprinting: an environmentally friendly approach for the development of separation methods for fingerprinting complex matrices

Citation

Funari, CS and Carneiro, RL and Andrade, AM and Hilder, EF and Cavalheiro, AJ, Green chromatographic fingerprinting: an environmentally friendly approach for the development of separation methods for fingerprinting complex matrices, Journal of Separation Science, 37, (1-2) pp. 37-44. ISSN 1615-9306 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Wiley-VCH Verlag

DOI: doi:10.1002/jssc.201300955

Abstract

A chromatographic fingerprint is a comprehensive method that reveals the distinctive pattern of peaks across the chromatogram for a given sample. It is considered an effective strategy to assess the identity and quality of herbal materials, as well as for the control of the quality of their derived products. HPLC is the most employed technique for these purposes and it is used routinely for quality control in industry. Hence, its impact on the environment should not be neglected. This work provides a rational and generic procedure to qualitatively fingerprint complex matrices. Resource- and time-saving experimental designs were selected; an alternative safer organic solvent was tested and a time-saving and innovative response entitled the green chromatographic fingerprinting response was developed and employed. This procedure was applied in the development of chromatographic fingerprints for extracts of Bauhinia forficata and Casearia sylvestris. Moreover, the response proposed here can be combined with a complementary metric available in the literature to compare methods using different solvents. According to this, the chromatographic fingerprints developed here using ethanol as the organic solvent provided a performance better than that of reference methods in which more harmful acetonitrile or methanol were employed.

Item Details

Item Type:Refereed Article
Keywords:Bauhinia forficata, Casearia sylvestris, quality by design, green liquid chromatography, metabolite profiling
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Funari, CS (Dr Cristiano Funari)
Author:Hilder, EF (Professor Emily Hilder)
ID Code:98603
Year Published:2014
Web of Science® Times Cited:11
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-20
Last Modified:2017-10-28
Downloads:0

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