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Simple and robust monitoring of ethanol fermentations by capillary electrophoresis

Citation

Oliver, JD and Sutton, AT and Karu, N and Phillips, M and Markham, J and Peiris, P and Hilder, EF and Castignolles, P, Simple and robust monitoring of ethanol fermentations by capillary electrophoresis, Biotechnology and Applied Biochemistry, 62, (3) pp. 329-342. ISSN 0885-4513 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 International Union of Biochemistry and Molecular Biology, Inc.

DOI: doi:10.1002/bab.1269

Abstract

Free-solution capillary electrophoresis (CE), or capillary zone electrophoresis, with direct UV detection was used for the first time for the determination of mono- and disaccharides, sugar alcohols, and ethanol in fermentation broths. Sample preparation proved to be minimal: no derivatization or specific sample purification was needed. The CE conditions can be adapted to the type of fermentation by simply altering the background electrolyte (BGE). KOH (130 mM) or NaOH (130 mM) as the BGE led to the fastest analysis time when monitoring simple fermentations. A mixture of 65 mM NaOH and 65 mM LiOH led to a 19% improvement in resolution for a complex mixture of carbohydrates. Quantification of a simple carbohydrate fermentation by CE showed values in close agreement with that of high-performance anion exchange chromatography and high-performance liquid chromatography (HPLC) on a cation exchange resin. For complex fermentations, quantification of carbohydrates by HPLC and CE led to similar results, whereas CE requires an injection volume of only 1020 nL. Analysis of an ethanol fermentation of hydrolyzed plant fiber demonstrated the robustness of the separation and detection of carbohydrates, as well as ethanol. Ethanol determination is achieved by coupling the CE method to pressure mobilization, using the same instrument and the same sample.

Item Details

Item Type:Refereed Article
Keywords:capillary electrophoresis, carbohydrate, fermentation, high performance anion exchange chromatography (HPAEC), high performance liquid chromatography (HPLC), resolution
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Karu, N (Dr Naama Karu)
Author:Hilder, EF (Professor Emily Hilder)
ID Code:98599
Year Published:2015
Funding Support:Australian Research Council (FT0990521)
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-19
Last Modified:2017-10-28
Downloads:0

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