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Comparative study of cytotoxicity of detonation nanodiamond particles with an osteosarcoma cell line and primary mesenchymal stem cells


Keremidarska, M and Ganeva, A and Mitev, D and Hikov, T and Presker, R and Pramatarova, L and Krasteva, N, Comparative study of cytotoxicity of detonation nanodiamond particles with an osteosarcoma cell line and primary mesenchymal stem cells, Biotechnology and Biotechnological Equipment, 28, (4) pp. 733-739. ISSN 1310-2818 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 The Author(s)

DOI: doi:10.1080/13102818.2014.947704


Recently, nanodiamonds (NDs) have attracted great interest due to their unique physical and chemical properties that could be used in various biological applications. However, depending on the origin, NDs often contain different impurities which may affect cellular functions and viability. Therefore, before their biomedical application, the cytotoxicity of newly produced NDs should be assessed.

In the present study, we have evaluated cytotoxicity of four types of ND particles with two cell models: a human osteosarcoma cell line, MG-63, and primary rat mesenchymal stem cells (rMSCs). Detonation-generated nanodiamond (DND) particles were purified with different acid oxidizers and impurities’ content was determined by elemental analysis. The particles size distribution was measured revealing that the DND particles have an average size in the range of 51–233 nm. Cytotoxicity was assessed by optical microscopy and proliferation assay after 72 hours exposure of the cells to nanoparticles. We observed cell-specific and material-specific toxicity for all tested particles. Primary stem cells demonstrated higher sensitivity to DND particles than osteosarcoma cells. The most toxic were the DND particles with the smallest grain size and slight content of non-diamond carbon, while DNDs with higher grain size and free from impurities had no significant influence on cell proliferation and morphology. In addition, the smaller DND particles were found to form large aggregates mainly during incubation with rMSCs. These results demonstrate the role of the purification method on the properties of DND particles and their cytotoxicity as well as the importance of cell types used for evaluation of the nanomaterials.

Item Details

Item Type:Refereed Article
Keywords:nanodiamond, cell proliferation, cell morphology, osteoblasts
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Mitev, D (Dr Dimitar Mitev)
ID Code:98579
Year Published:2014
Web of Science® Times Cited:25
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-19
Last Modified:2015-08-06

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