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Development of nuclear microsatellite markers for the Tasmanian endemic conifer Diselma archeri Hook. F. (Cupressaceae)

Citation

Sakaguchi, S and Worth, JRP and Jordan, GJ and Thomas, P and Ito, M, Development of nuclear microsatellite markers for the Tasmanian endemic conifer Diselma archeri Hook. F. (Cupressaceae), Conservation Genetics Resources, 7, (2) pp. 369-372. ISSN 1877-7252 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 Springer Science+Business Media

DOI: doi:10.1007/s12686-014-0368-4

Abstract

Novel microsatellite markers were developed for the Tasmanian endemic conifer Cheshunt Pine (Diselma archeri), a species vulnerable to increased fire frequency under warming and drying climates. Ten polymorphic microsatellite markers were developed and characterized. The number of alleles per locus ranged from 5 to 14, and average observed heterozygosity was 0.756. No significant departures from Hardy–Weinberg equilibrium were detected at all loci. These markers will be useful to investigate population genetics and the importance of clonality for reproduction, for better understandings of the species’ history and ecology.

Item Details

Item Type:Refereed Article
Keywords:Cupressaceae, Diselma archeri, Next generation sequencing, Tasmanian endemic, microsatellites, conifers, gymnosperm
Research Division:Biological Sciences
Research Group:Evolutionary Biology
Research Field:Biogeography and Phylogeography
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Worth, JRP (Dr James Worth)
Author:Jordan, GJ (Associate Professor Greg Jordan)
ID Code:98532
Year Published:2015 (online first 2014)
Funding Support:Australian Research Council (DP120100501)
Deposited By:Plant Science
Deposited On:2015-02-18
Last Modified:2017-11-01
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