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High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach


Tubaon, RM and Haddad, PR and Quirino, JP, High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach, Journal of Chromatography A, 1349 pp. 129-134. ISSN 0021-9673 (2014) [Refereed Article]

Copyright Statement

Crown Copyright İ 2014 Published by Elsevier B.V.

DOI: doi:10.1016/j.chroma.2014.05.007


A synergistic stacking approach whereby field-enhanced sample injection and micelle-to-solvent stacking in capillary zone electrophoresis are combined has been developed and has been applied to the separation and quantification of anionic sulfonamides. Electrokinetic injection of the sample in a low conductivity alkaline diluent was performed for 90 s at −15 kV. Micelle-to-solvent stacking was then undertaken by hydrodynamic injection of micellar cetyltrimethylammonium bromide solution prior to the electrokinetic injection of sample that also contained 50% methanol. This combined stacking approach, when compared to a typical hydrostatic injection, provided improvements in peak height and corrected peak area in the range of 397–1024 and 758–1246, respectively. Limits of quantification in the range of 0.01–0.03 μg/mL were obtained for sulfamerazine, sulfamethazine and sulfamethizole and were sufficient for the determination of these analytes in river water. The percentage recovery and accuracy values obtained for a fortified river water sample that had been subjected to sample preparation by evaporation and reconstitution with diluent were 74–135%. Intra-day and inter-day repeatabilities for migration time, peak height, and corrected peak area were in the range 0.5–5.0% (percentage relative standard deviation, n = 8) and these relatively low values were attributed to the use of a stable capillary coating established by the successive multiple ionic-polymer layer technique.

Item Details

Item Type:Refereed Article
Keywords:field-enhanced sample injection, micelle-to-solvent stacking, sulfonamides, capillary zone electrophoresis, successive multiple ionic-polymer layer, river water
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Tubaon, RM (Ms Ria Tubaon)
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:98473
Year Published:2014
Web of Science® Times Cited:20
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-17
Last Modified:2017-10-27

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