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High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach
journal contribution
posted on 2023-05-18, 07:29 authored by Ria Amuno, Paul HaddadPaul Haddad, Joselito QuirinoA synergistic stacking approach whereby field-enhanced sample injection and micelle-to-solvent stacking in capillary zone electrophoresis are combined has been developed and has been applied to the separation and quantification of anionic sulfonamides. Electrokinetic injection of the sample in a low conductivity alkaline diluent was performed for 90 s at −15 kV. Micelle-to-solvent stacking was then undertaken by hydrodynamic injection of micellar cetyltrimethylammonium bromide solution prior to the electrokinetic injection of sample that also contained 50% methanol. This combined stacking approach, when compared to a typical hydrostatic injection, provided improvements in peak height and corrected peak area in the range of 397–1024 and 758–1246, respectively. Limits of quantification in the range of 0.01–0.03 μg/mL were obtained for sulfamerazine, sulfamethazine and sulfamethizole and were sufficient for the determination of these analytes in river water. The percentage recovery and accuracy values obtained for a fortified river water sample that had been subjected to sample preparation by evaporation and reconstitution with diluent were 74–135%. Intra-day and inter-day repeatabilities for migration time, peak height, and corrected peak area were in the range 0.5–5.0% (percentage relative standard deviation, n = 8) and these relatively low values were attributed to the use of a stable capillary coating established by the successive multiple ionic-polymer layer technique.
History
Publication title
Journal of Chromatography AVolume
1349Pagination
129-134ISSN
0021-9673Department/School
School of Natural SciencesPublisher
Elsevier Science BvPlace of publication
PO Box 211, Amsterdam, Netherlands, 1000 AeRights statement
Crown Copyright © 2014 Published by Elsevier B.V.Repository Status
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