A synergistic stacking approach whereby field-enhanced sample injection and micelle-to-solvent stacking in capillary zone electrophoresis are combined has been developed and has been applied to the separation and quantification of anionic sulfonamides. Electrokinetic injection of the sample in a low conductivity alkaline diluent was performed for 90 s at −15 kV. Micelle-to-solvent stacking was then undertaken by hydrodynamic injection of micellar cetyltrimethylammonium bromide solution prior to the electrokinetic injection of sample that also contained 50% methanol. This combined stacking approach, when compared to a typical hydrostatic injection, provided improvements in peak height and corrected peak area in the range of 397–1024 and 758–1246, respectively. Limits of quantification in the range of 0.01–0.03 μg/mL were obtained for sulfamerazine, sulfamethazine and sulfamethizole and were sufficient for the determination of these analytes in river water. The percentage recovery and accuracy values obtained for a fortified river water sample that had been subjected to sample preparation by evaporation and reconstitution with diluent were 74–135%. Intra-day and inter-day repeatabilities for migration time, peak height, and corrected peak area were in the range 0.5–5.0% (percentage relative standard deviation, n = 8) and these relatively low values were attributed to the use of a stable capillary coating established by the successive multiple ionic-polymer layer technique.

Item Type:	Refereed Article
Keywords:	field-enhanced sample injection, micelle-to-solvent stacking, sulfonamides, capillary zone electrophoresis, successive multiple ionic-polymer layer, river water
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Tubaon, RM (Ms Ria Tubaon)
UTAS Author:	Haddad, PR (Professor Paul Haddad)
UTAS Author:	Quirino, JP (Associate Professor Lito Quirino)
ID Code:	98473
Year Published:	2014
Web of Science® Times Cited:	19
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2015-02-17
Last Modified:	2017-10-27
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