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High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach
Citation
Tubaon, RM and Haddad, PR and Quirino, JP, High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach, Journal of Chromatography A, 1349 pp. 129-134. ISSN 0021-9673 (2014) [Refereed Article]
Copyright Statement
Crown Copyright İ 2014 Published by Elsevier B.V.
DOI: doi:10.1016/j.chroma.2014.05.007
Abstract
A synergistic stacking approach whereby field-enhanced sample injection and micelle-to-solvent stacking in capillary zone electrophoresis are combined has been developed and has been applied to the separation and quantification of anionic sulfonamides. Electrokinetic injection of the sample in a low conductivity alkaline diluent was performed for 90 s at −15 kV. Micelle-to-solvent stacking was then undertaken by hydrodynamic injection of micellar cetyltrimethylammonium bromide solution prior to the electrokinetic injection of sample that also contained 50% methanol. This combined stacking approach, when compared to a typical hydrostatic injection, provided improvements in peak height and corrected peak area in the range of 3971024 and 7581246, respectively. Limits of quantification in the range of 0.010.03 μg/mL were obtained for sulfamerazine, sulfamethazine and sulfamethizole and were sufficient for the determination of these analytes in river water. The percentage recovery and accuracy values obtained for a fortified river water sample that had been subjected to sample preparation by evaporation and reconstitution with diluent were 74135%. Intra-day and inter-day repeatabilities for migration time, peak height, and corrected peak area were in the range 0.55.0% (percentage relative standard deviation, n = 8) and these relatively low values were attributed to the use of a stable capillary coating established by the successive multiple ionic-polymer layer technique.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | field-enhanced sample injection, micelle-to-solvent stacking, sulfonamides, capillary zone electrophoresis, successive multiple ionic-polymer layer, river water |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Tubaon, RM (Ms Ria Tubaon) |
| Author: | Haddad, PR (Professor Paul Haddad) |
| Author: | Quirino, JP (Associate Professor Lito Quirino) |
| ID Code: | 98473 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 14 |
| Deposited By: | Austn Centre for Research in Separation Science |
| Deposited On: | 2015-02-17 |
| Last Modified: | 2017-10-27 |
| Downloads: | 0 |
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