eCite Digital Repository

High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach

Citation

Tubaon, RM and Haddad, PR and Quirino, JP, High-sensitivity analysis of anionic sulfonamides by capillary electrophoresis using a synergistic stacking approach, Journal of Chromatography A, 1349 pp. 129-134. ISSN 0021-9673 (2014) [Refereed Article]

Copyright Statement

Crown Copyright İ 2014 Published by Elsevier B.V.

DOI: doi:10.1016/j.chroma.2014.05.007

Abstract

A synergistic stacking approach whereby field-enhanced sample injection and micelle-to-solvent stacking in capillary zone electrophoresis are combined has been developed and has been applied to the separation and quantification of anionic sulfonamides. Electrokinetic injection of the sample in a low conductivity alkaline diluent was performed for 90 s at −15 kV. Micelle-to-solvent stacking was then undertaken by hydrodynamic injection of micellar cetyltrimethylammonium bromide solution prior to the electrokinetic injection of sample that also contained 50% methanol. This combined stacking approach, when compared to a typical hydrostatic injection, provided improvements in peak height and corrected peak area in the range of 397–1024 and 758–1246, respectively. Limits of quantification in the range of 0.01–0.03 μg/mL were obtained for sulfamerazine, sulfamethazine and sulfamethizole and were sufficient for the determination of these analytes in river water. The percentage recovery and accuracy values obtained for a fortified river water sample that had been subjected to sample preparation by evaporation and reconstitution with diluent were 74–135%. Intra-day and inter-day repeatabilities for migration time, peak height, and corrected peak area were in the range 0.5–5.0% (percentage relative standard deviation, n = 8) and these relatively low values were attributed to the use of a stable capillary coating established by the successive multiple ionic-polymer layer technique.

Item Details

Item Type:Refereed Article
Keywords:field-enhanced sample injection, micelle-to-solvent stacking, sulfonamides, capillary zone electrophoresis, successive multiple ionic-polymer layer, river water
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Tubaon, RM (Ms Ria Tubaon)
Author:Haddad, PR (Professor Paul Haddad)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:98473
Year Published:2014
Web of Science® Times Cited:13
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-17
Last Modified:2017-10-27
Downloads:0

Repository Staff Only: item control page