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Anionic microemulsion to solvent stacking for on-line sample concentration of cationic analytes in capillary electrophoresis


Kukusamude, C and Srijaranai, S and Quirino, JP, Anionic microemulsion to solvent stacking for on-line sample concentration of cationic analytes in capillary electrophoresis, Electrophoresis, 35, (10) pp. 1478-1483. ISSN 0173-0835 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Wiley-VCH Verlag

DOI: doi:10.1002/elps.201300512


The common SDS microemulsion (i.e. 3.3% SDS, 0.8% octane, and 6.6% butanol) and organic solvents were investigated for the stacking of cationic drugs in capillary zone electrophoresis using a low pH separation electrolyte. The sample was prepared in the acidic microemulsion and a high percentage of organic solvent was included in the electrolyte at anodic end of capillary. The stacking mechanism was similar to micelle to solvent stacking where the micelles were replaced by the microemulsion for the transport of analytes to the organic solvent rich boundary. This boundary is found between the microemulsion and anodic electrolyte. The effective electrophoretic mobility of the cations reversed from the direction of the anode in the microemulsion to the cathode in the boundary. Microemulsion to solvent stacking was successfully achieved with 40% ACN in the anodic electrolyte and hydrodynamic sample injection of 21 s at 1000 mbar (equivalent to 30% of the effective length). The sensitivity enhancement factors in terms of peak height and corrected peak area were 15 to 35 and 21 to 47, respectively. The linearity R2 in terms of corrected peak area were >0.999. Interday precisions (%RSD, n = 6) were 3.34.0% for corrected peak area and 2.03.0% for migration time. Application to spiked real sample is also presented.

Item Details

Item Type:Refereed Article
Keywords:capillary electrophoresis, microemulsion to solvent stacking, on-line sample concentration
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Kukusamude, C (Mr Chunyapuk Kukusamude)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:98470
Year Published:2014
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-17
Last Modified:2018-03-16

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