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Micellar electrokinetic chromatography of the constituents in Philippine lagundi (Vitex negundo) herbal products

Citation

Rabanes, HR and Guidote Jr, AM and Quirino, JP, Micellar electrokinetic chromatography of the constituents in Philippine lagundi (Vitex negundo) herbal products, Microchemical Journal, 112 pp. 153-158. ISSN 0026-265X (2014) [Refereed Article]

Copyright Statement

Crown Copyright 2013

DOI: doi:10.1016/j.microc.2013.10.006

Abstract

Vitex negundo or lagundi is an important herbal medicine in Asia and various commercial products of this herb's leaves have been flooding the market. Reports on the chemical constituents of such products were limited and directed to only one selected chemical marker. Here, micellar electrokinetic chromatography using a fused silica capillary was used for analysis of the methanolic extract of Philippine lagundi herbal products. The separation solution, injection, and separation voltage was 50 mM SDS with 50 mM phosphoric acid at pH 2.5, 6 s at 25 mbar, and 20 kV at negative polarity, respectively. With UV detection at 200 nm, the LODs (S/N = 3) were in the sub-μg/mL range which were sufficient for detection without the need for on-line or off-line sample concentration. The repeatability values were all below 3.9% for migration time and peak signals. When the method was used to real samples, a flavonoid (isoorientin) and two iridoid glycosides (agnuside and negundoside) were identified as the target chemical markers within a 12 min electrophoretic run. The accuracy was from 95% to 129%. The results also suggested that agnuside was the major component in Philippine lagundi. The method was simple, effective, low cost, and can be used for authenticity evaluation of lagundi products.

Item Details

Item Type:Refereed Article
Keywords:Vitex negundo, lagundi, MEKC, chemical markers, herbal products, agnuside
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Manufacturing
Objective Group:Human Pharmaceutical Products
Objective Field:Human Pharmaceutical Treatments (e.g. Antibiotics)
Author:Rabanes, HR (Ms Heide Rabanes)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:98465
Year Published:2014
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2015-02-17
Last Modified:2017-10-27
Downloads:0

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