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Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1 with grain yield for increasing salinity tolerance in barley
Citation
Liu, X and Mak, M and Babla, M and Wang, F and Chen, G and Veljanoski, F and Wang, G and Shabala, S and Zhou, M and Chen, Z-H, Linking stomatal traits and expression of slow anion channel genes HvSLAH1 and HvSLAC1 with grain yield for increasing salinity tolerance in barley, Frontiers in Plant Science, 5 Article 634. ISSN 1664-462X (2014) [Refereed Article]
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Copyright Statement
Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
DOI: doi:10.3389/fpls.2014.00634
Abstract
Soil salinity is an environmental and agricultural problem in many parts of the world. One of the keys to breeding barley for adaptation to salinity lies in a better understanding of the genetic control of stomatal regulation. We have employed a range of physiological (stomata assay, gas exchange, phylogenetic analysis, QTL analysis), and molecular techniques (RT-PCR and qPCR) to investigate stomatal behavior and genotypic variation in barley cultivars and a genetic population in four experimental trials. A set of relatively efficient and reliable methods were developed for the characterization of stomatal behavior of a large number of varieties and genetic lines. Furthermore, we found a large genetic variation of gas exchange and stomatal traits in barley in response to salinity stress. Salt-tolerant cultivar CM72 showed significantly larger stomatal aperture under 200 mM NaCl treatment than that of salt-sensitive cultivar Gairdner. Stomatal traits such as aperture width/length were found to significantly correlate with grain yield under salt treatment. Phenotypic characterization and QTL analysis of a segregating double haploid population of the CM72/Gairdner resulted in the identification of significant stomatal traits-related QTLs for salt tolerance. Moreover, expression analysis of the slow anion channel genes HvSLAH1 and HvSLAC1 demonstrated that their up-regulation is linked to higher barley grain yield in the field.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | barley, soil salinity, stomata, gas exchange, quantitative trait loci, HvSLAC1, HvSLAH1, Hordeum vulgare L |
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Crop and pasture production |
| Research Field: | Crop and pasture improvement (incl. selection and breeding) |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Wang, F (Miss Feifei Wang) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| ID Code: | 98433 |
| Year Published: | 2014 |
| Web of Science® Times Cited: | 36 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2015-02-16 |
| Last Modified: | 2017-11-02 |
| Downloads: | 447 View Download Statistics |
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