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Receptor kinase-mediated control of primary active proton pumping at the plasma membrane

Citation

Fuglsang, AT and Kristensen, A and Cuin, TA and Schulze, WX and Persson, J and Thuesen, KH and Ytting, CK and Oehlenschlaeger, CB and Mahmood, K and Sondergaard, TE and Shabala, S and Palmgren, MG, Receptor kinase-mediated control of primary active proton pumping at the plasma membrane, The Plant Journal, 80, (6) pp. 951-964. ISSN 0960-7412 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 John Wiley & Sons Ltd

DOI: doi:10.1111/tpj.12680

Abstract

Acidification of the cell wall space outside the plasma membrane is required for plant growth and is the result of proton extrusion by the plasma membrane-localized H+-ATPases. Here we show that the major plasma membrane proton pumps in Arabidopsis, AHA1 and AHA2, interact directly in vitro and in planta with PSY1R, a receptor kinase of the plasma membrane that serves as a receptor for the peptide growth hormone PSY1. The intracellular protein kinase domain of PSY1R phosphorylates AHA2/AHA1 at Thr-881, situated in the autoinhibitory region I of the C-terminal domain. When expressed in a yeast heterologous expression system, the introduction of a negative charge at this position caused pump activation. Application of PSY1 to plant seedlings induced rapid in planta phosphorylation at Thr-881, concomitant with an instantaneous increase in proton efflux from roots. The direct interaction between AHA2 and PSY1R observed might provide a general paradigm for regulation of plasma membrane proton transport by receptor kinases.

Item Details

Item Type:Refereed Article
Keywords:LRR kinase, H+ pump, peptide signalling, apoplastic pH, cell elongation
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Cuin, TA (Dr Tracey Cuin)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:98156
Year Published:2014
Web of Science® Times Cited:19
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-02-03
Last Modified:2017-11-02
Downloads:0

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